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Determination of MHV RNA poly(A) tail length and 3' end additions upon NCTC cells infection

ABSTRACT: The mouse hepatitis virus (MHV) genomic and sub-genomic RNAs have 3’ poly(A) tails. The terminal addition of uridines to poly(A) tails has been shown to be a widespread modification. Here, we investigated the presence of 3' end additions on the MHV RNA poly(A) tails. To this end, we infected NCTC cells with MHV and isolated RNA at 24-hours post-infection (hpi). While the median poly(A) tail length of the MHV RNAs is around 50 nucleotides, we observed a peak of uridylation in transcripts with poly(A) tails about 40 nucleotides long

ORGANISM(S): Mus musculus

PROVIDER: GSE200414 | GEO | 2023/03/23

REPOSITORIES: GEO

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Project description:This SuperSeries is composed of the SubSeries listed below.

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