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Long non-coding RNA GDAR regulate ovine granulosa cells apoptosis by affecting the expression of apoptosis-related genes

ABSTRACT: Short-term dietary supplementation of female sheep during the luteal phase can increase fertility and most probably by stimulating glucose uptake by the follicles. However, the molecular mechanism of glucose regulates follicular development has not yet been clarified, especially the further study of long non-coding RNA (lncRNA) in determining fertility during follicular development. We generated GCs models of different doses of glucose (0, 2.1, 4.2, 8.4, 16.8 and 33.6 mM), and observed that the highest cell viability was recorded in the 8.4 mM group and the highest apoptosis rates were recorded in the 33.6 mM groups. Therefore, the control (n = 3, 0 mM glucose group), low glucose (n = 3, add 8.4 mM glucose concentration group), and high glucose (n = 3, add 33.6 mM glucose concentration group) of GCs were created for the next whole genomic RNA sequencing (RNA-seq). In total, 11,221 novel lncRNAs and 510 annotated lncRNAs were identified in the GCs samples. Gene ontology term enrichment indicates the obvious enrichment in apoptosis and its related pathways, which suggests that cell apoptosis could play a critical role in the process of glucose-induced GCs differentiation. Furthermore, we focused on the function of a lncGDAR and verified that lncGDAR could involve cell apoptosis by effecting the expression of Bcl-2, BAX, Caspase-3, and Caspase-7 genes. These results provide the basis for further study of LncRNA regulation mechanism in nutrition on female fertility.

ORGANISM(S): Ovis aries

PROVIDER: GSE200668 | GEO | 2022/05/18

REPOSITORIES: GEO

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