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Gene expression profiles during the process of differentiation of HL-60 cells into neutrophils or eosinophils

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE200827 | GEO | 2022/12/28

REPOSITORIES: GEO

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Gene expression profiles during the process of differentiation of HL-60 cells into neutrophils [HL60]

Project description:To investigate the changes in gene expression during the process of differentiation into neutrophils, microarray analysis was performed. HL-60 cells were cultured with all-trans retinoic acid (ATRA) for 4 days. The cells were collected at four different time points and total RNA was prepared for use as a sample for microarray analysis.

2022-12-28 | GSE200823 | GEO

Gene expression profiles during the process of differentiation of HL-60 clone 15 cells into eosinophils [HL60cl15]

Project description:To investigate the changes in gene expression during the process of differentiation into eosinophils, microarray analysis was performed. HL-60 clone 15 cells were cultured with apicidin for 6 days. The cells were collected at four different time points and total RNA was prepared for use as a sample for microarray analysis.

2022-12-28 | GSE200826 | GEO

Gene expression profiles during the process of differentiation of HL-60 cells into neutrophils or eosinophils

Project description:Gene expression profiles during the process of differentiation of HL-60 cells into neutrophils or eosinophils

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Gene expression profiles during the process of differentiation of HL-60 AK2 hetero-knockout clone #47 cells into neutrophils [HL60AK2KO_clone47]

Project description:To investigate the changes in gene expression during the process of differentiation into neutrophils and to evaluate the effect of Ak2 hetero-knockout in the process, microarray analysis was performed. HL-60 Ak2 hetero-knockout clone #47 cells were cultured with all-trans retinoic acid (ATRA) for 4 days. The cells were collected at four different time points and total RNA was prepared for use as a sample for microarray analysis.

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Gene expression profiles during the process of differentiation of HL-60 AK2 hetero-knockout clone #5 cells into neutrophils [HL60AK2KO_clone5]

Project description:To investigate the changes in gene expression during the process of differentiation into neutrophils and to evaluate the effect of Ak2 hetero-knockout in the process, microarray analysis was performed. HL-60 Ak2 hetero-knockout clone #5 cells were cultured with all-trans retinoic acid (ATRA) for 4 days. The cells were collected at four different time points and total RNA was prepared for use as a sample for microarray analysis.

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Gene expression profiles during the process of differentiation of HL-60 cells into neutrophils [HL60]

Project description:Gene expression profiles during the process of differentiation of HL-60 cells into neutrophils [HL60]

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Gene expression profiles during the process of differentiation of HL-60 clone 15 cells into eosinophils [HL60cl15]

Project description:Gene expression profiles during the process of differentiation of HL-60 clone 15 cells into eosinophils [HL60cl15]

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Deacetylase activity-independent transcriptional activation by HDAC2 during TPA-induced HL-60 cell differentiation.

Project description:The human myeloid leukemia cell line HL-60 differentiate into monocytes following treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA). However, the mechanism underlying the differentiation of these cells in response to TPA has not been fully elucidated. In this study, we performed ChIP-seq profiling of RNA Pol II, HDAC2, Acetyl H3 (AcH3), and H3K27me3 and analyzed differential chromatin state changes during TPA-induced differentiation of HL-60 cells. We focused on atypically active genes, which showed enhanced H3 acetylation despite increased HDAC2 recruitment. We found that HDAC2 positively regulates the expression of these genes in a histone deacetylase activity-independent manner. HDAC2 interacted with and recruited paired box 5 (PAX5) to the promoters of the target genes and regulated HL-60 cell differentiation by PAX5-mediated gene activation. Taken together, these data elucidated the specific-chromatin status during HL-60 cell differentiation following TPA exposure and suggested that HDAC2 can activate transcription of certain genes through interactions with PAX5 in a deacetylase activity-independent pathway.

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An efficient strategy to identify early TPA-responsive genes during differentiation of HL-60 cells.

Project description:We have adopted a special experimental strategy to identify early responsive genes during 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced macrophage-like differentiation of human myeloid leukemia cells (HL-60). This was performed in cells that were synchronized by nocodazole and treated with TPA in the presence of a protein synthesis inhibitor, cycloheximide, to prevent activation of secondary targets and therefore increase the probability of early transcripts in total RNA pool. The expression alteration was analyzed by microarray and the selection criteria of candidate genes were adjusted by real-time PCR validation to increase its reliability. Finally, 56 genes were identified as early TPA-responsive genes in this multiscreening step approach. Furthermore, upregulation of three candidate genes (NFIL3, SKIL, and JMJD3) was shown to be dosage and time dependent with TPA treatment and was found to be directly regulated by TPA through PKC-dependent signaling. These results revealed that our screenings provide a useful and efficient approach to identify early TPA-responsive genes and these genes might involve the regulation of TPA-induced differentiation program of HL-60 cells as primary

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HL-60 cells following treatment with different compounds

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-11-26 | GSE124423 | GEO