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5PSeq supporting study Probing 3’UTRs as modular regulators of gene expression

ABSTRACT: The goal of the study is to analyse the function of 3'-UTR motifs in the non-native context. Several 3'-UTR motifs predicted to have stabilizing or destabilizing effects on mRNA were inserted into terminators controlling the expression of reporter mCherry gene in following contexts: pRps3-mCherry-tRps3, pPgk1-mCherry-tRps3, pTsa1-mCherry-tTsa. 5'P sequencing was used to determine whether or not modifications in the 3'-UTR region affect ribosomal dynamics and co-translational degradation of mRNA in the 3' end region of reporter coding sequence.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE200950 | GEO | 2022/04/23

REPOSITORIES: GEO

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QuantSeq/3' targeted seq supporting study Probing 3’UTRs as modular regulators of gene expression

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