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QuantSeq/3' targeted seq supporting study Probing 3’UTRs as modular regulators of gene expression

ABSTRACT: The goal of the study is to analyze the function of 3'-UTR motifs in the non-native context. Several 3'-UTR motifs predicted to have stabilizing or destabilizing effects on mRNA were inserted into terminators controlling the expression of reporter mCherry gene in following contexts: pRps3-mCherry-tRps3, pPgk1-mCherry-tRps3, pTsa1-mCherry-tTsa. 3'-end sequencing was used to measure the levels of expression of reporter gene and distribution of poly(A) sites.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE200211 | GEO | 2022/04/09

REPOSITORIES: GEO

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5PSeq supporting study Probing 3’UTRs as modular regulators of gene expression

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