Transcriptomics

Dataset Information

0

Gene expression profile of yeast cells under stress [bulk RNA-seq]


ABSTRACT: To understand transcriptomic changes in baker’s yeast S. cerevisiae cells under stress conditions, we sequenced 117 yeast cells under three stress treatments (hypotonic condition, glucose starvation and amino acid starvation) using a full-length based single-cell RNA-Seq method. We found that though single cells from the same treatment showed varying degrees of uniformity, technical noise and batch effects can confound results significantly. However, upon careful selection of samples to reduce technical artifacts and account for batch-effects, we were able to capture distinct transcriptomic signatures for different stress conditions. Our results show that a full single cell based transcriptomic analysis of the yeast may help paint a clearer picture of how the model organism responds to stress than do bulk cell population-based methods.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE201385 | GEO | 2022/04/26

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2022-04-26 | GSE201386 | GEO
2008-06-25 | GSE6454 | GEO
2009-07-31 | GSE8673 | GEO
2017-07-11 | GSE101071 | GEO
2017-01-30 | E-MTAB-3459 | biostudies-arrayexpress
2006-03-07 | E-GEOD-4398 | biostudies-arrayexpress
2011-05-15 | GSE29046 | GEO
2006-03-08 | GSE4398 | GEO
2011-09-21 | E-GEOD-12962 | biostudies-arrayexpress
2014-07-12 | E-GEOD-59353 | biostudies-arrayexpress