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In vivo self-assembly and targeted delivery of EGFR siRNA-encapsulating small extracellular vesicles to EGFR-positive cancer cells for NSCLC therapy

ABSTRACT: We designed a synthetic biology circuit encoding both an EGFR-targeting peptide (GE11) and an EGFR siRNA under the control of the cytomegalovirus (CMV) promoter. After uptake by mouse liver cells via intravenous injection, this genetic circuit can reprogram the liver cells to produce and self-assemble EGFR siRNAs into secretory small extracellular vesicles (sEVs) tagged with the GE11 peptide, after which the siRNA-encapsulating sEVs are further transported via the blood circulation and guided by the GE11 peptide to EGFR-positive tumour cells for NSCLC therapy. RNA sequencing-based transcriptomic profiling confirmed the safety and low off-target activity of in vivo self-assembled EGFR siRNAs.

ORGANISM(S): Mus musculus

PROVIDER: GSE201969 | GEO | 2024/04/30

REPOSITORIES: GEO

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In vivo self-assembly and targeted delivery of EGFR siRNA-encapsulating small extracellular vesicles to EGFR-positive cancer cells for NSCLC therapy

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