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Decoding the m6A expression profiling of spinal cord after spared nerve injury at three time points with RIP-Seq

ABSTRACT: We performed gene expression profiling analysis using data obtained from RNA-seq and m6A peaks profiling analysis using data obtained from RIP-Seq of spared nerve injury model at 3rd, 7th, 14th postoperative day and sham group. To investigate the differential m6A expression profiling, we established spared nerve injury model at 3rd, 7th, 14th postoperative day and sham group.

ORGANISM(S): Mus musculus

PROVIDER: GSE202166 | GEO | 2022/05/06

REPOSITORIES: GEO

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Small extracellular vesicles from spared nerve injury model and sham control mice differentially regulate gene expression in primary microglia

Project description:Nerve injury outcomes might be predicted by examining small extracellular vesicles (sEVs) in circulation, as their biomolecular cargo facilitates cellular communication and can alter transcriptional state and behavior of recipient cells. We found that sEVs from the serum of spared nerve injury (SNI) model male mice had seven differentially expressed miRNAs compared to sEVs from sham-operated control mice four weeks post-surgery. We investigated how these sEVs alter transcription in primary cortical microglia, a crucial mediator of neuropathic pain, using RNA sequencing. While the uptake of sEVs from both SNI model and sham groups changed gene expression in microglia compared to PBS treatment, sEVs from the sham group induced a more drastic change, particularly in genes involved in immune response. This was recapitulated by increased levels of pro-inflammatory cytokines and chemokines in microglia incubated with sEVs from sham control compared to sEVs from SNI model, naïve mice, or PBS. However, treating microglia with sEVs from female mice showed that serum sEVs derived from female SNI mice but not from female sham mice induced a more pronounced microglial secretion of pro-inflammatory mediators. Our data demonstrate that the molecular changes induced by sham surgery injuring skin and muscles are reflected in circulating sEVs in male mice four weeks later. Thus, when using sEVs from sham mice as control in comparative mechanistic studies after nerve injury, sex of mice should be taken into consideration.

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Desipramine Induces Anti-inflammatory Dorsal Root Ganglion Transcriptional Signatures in the Murine Spared Nerve Injury Model

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Decoding the m6A expression profiling of spinal cord after spared nerve injury at three time points with RIP-Seq

Project description:Decoding the m6A expression profiling of spinal cord after spared nerve injury at three time points with RIP-Seq

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Transcriptome changes in the dorsal root ganglion (DRG) after spared nerve injury (SNI) in adult and infant rats.

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Small extracellular vesicles from spared nerve injury model and sham control mice differentially regulate gene expression in primary microglia

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