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Transcription profiling of E. coli cultures grown on different carbon sources

ABSTRACT: The study investigated the effect of different carbon sources on E. coli global gene expression. We grew MG1655 cells aerobically in MOPS minimal medium with either glucose, glycerol, succinate, L-alanine, acetate, or L-proline as the carbon supply. Samples were taken from each culture at mid log phase and were RNA-stabilized using Qiagen RNAProtect Bacterial Reagent (Qiagen). Total RNA was then isolated using MasterPure kits (Epicentre Technologies). Purified RNA was reverse-transcribed to cDNA, labeled and hybridized to Affymetrix GeneChipÃ E. coli Antisense Genome Arrays as recommended in the technical manual (www.affymetrix.com).

ORGANISM(S): Escherichia coli

SUBMITTER: Mingzhu Liu

PROVIDER: E-GEOD-2037 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Global transcriptional programs reveal a carbon source foraging strategy by Escherichia coli.

Liu Mingzhu M Durfee Tim T Cabrera Julio E JE Zhao Kai K Jin Ding J DJ Blattner Frederick R FR

The Journal of biological chemistry 20050210 16

By exploring global gene expression of Escherichia coli growing on six different carbon sources, we discovered a striking genome transcription pattern: as carbon substrate quality declines, cells systematically increase the number of genes expressed. Gene induction occurs in a hierarchical manner and includes many factors for uptake and metabolism of better but currently unavailable carbon sources. Concomitantly, cells also increase their motility. Thus, as the growth potential of the environmen ...[more]

PMID: 15705577

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