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Expression data from Transgenic mice skin expressing deltaNp63alpha

ABSTRACT: We developed a Tet-inducible system to express deltaNp63alpha isoform under the control of keratin 5 promoter. Transgenic mice, which were Bigenic (BG) developed a severe skin phenotype with abnormal keratinocyte differentiation and defects in hair follicle development and cycling. Skin samples from transgenic animals and wild type animals were analyzed for global transcriptome changes.

ORGANISM(S): Mus musculus

PROVIDER: GSE20514 | GEO | 2010/04/16

SECONDARY ACCESSION(S): PRJNA125235

REPOSITORIES: GEO

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Expression data from Transgenic mice skin expressing deltaNp63alpha

Project description:We developed a Tet-inducible system to express deltaNp63alpha isoform under the control of keratin 5 promoter. Transgenic mice, which were Bigenic (BG) developed a severe skin phenotype with abnormal keratinocyte differentiation and defects in hair follicle development and cycling. Skin samples from transgenic animals and wild type animals were analyzed for global transcriptome changes. We used microarrays to performing transcriptional profiling of deltaNp63alpha transgenic and control dorsal skin at P16.

2010-04-28 | E-GEOD-20514 | biostudies-arrayexpress

CGH Analysis of Murine Squamous Cell Carcinoma: Control vs. Tumors overexpressing Aurora Kinase A

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Mus musculus

Project description:Expression data from Transgenic mice skin expressing deltaNp63alpha

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Effect of enzymatically-hydrolysed eggshell membrane (eESM) in Human keratinocyte

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Transcription profiling of mouse FVB/N wild type and transgenic Tg.AC mice to examine skin papilloma development

Project description:This study analyzes gene expression associated with papilloma development in Tg.AC v-Ha-ras transgenic mice and identifies novel genes and biological processes that may be critical to skin carcinogenesis in these mice. Epidermal abrasion was used to synchronously induce epidermal regeneration in FVB/N wild type and transgenic Tg.AC mice. Skin papillomagenesis was uniquely induced in Tg.AC mice, and gene expression profiling was carried out using a 22,000 element mouse DNA microarray. Histological analysis showed that papillomas developed at a high rate by day 30 after abrasion in transgenic animals, while no papilloma developed in wild type mice. Transgene specific differentially expressed genes were identified at day 30 post-abrasion and these genes were annotated using EASE software and literature mining. Annotated and non-annotated genes associated with papilloma development were identified and clustering analysis revealed groups of genes that are coordinately expressed. A number of genes associated with differentiation and development were also physically clustered on mouse chromosome 16, including 16B3 that contains several Stefins and stefin-like genes, and 16A1 containing a number of keratin associated protein genes. Additional analyses presented here yield novel insights into the genes and processes involved in papilloma development in Tg.AC mice. Experiment Overall Design: Strains: Tg.AC vs FVB/N; Treatment: epidermal abrasion vs sham treatment, samples collected 3, 5, 9, 18, and 30 days post treatment; Microarray: 2-channel, pooled total RNA from 5 animals, 2 technical replicate arrays with dye-swaps.

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Expression data from K5-IKKbeta transgenic mouse skin

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Identification of Genes and Gene Ontology Process Critical to Skin Papilloma Development in Tg.AC Transgenic Mice

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2008-04-11 | GSE11136 | GEO

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2017-01-02 | PXD001645 | Pride

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