Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Investigation of differential expression: Differentiating cells vs Salmonella infected cells (t6)

ABSTRACT: Dictyostelium discoideum is a professional phagocyte and it uses phagocytosis as a way to get food. However, there are a number of bacteria that subvert this professional phagocyte. In this study we investigated whether Salmonella typhimurium is also pathogenic for D. discoideum.

ORGANISM(S): Dictyostelium discoideum

PROVIDER: GSE20648 | GEO | 2010/03/10

SECONDARY ACCESSION(S): PRJNA129609

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Investigation of differential expression: Differentiating cells vs Salmonella infected cells (t3)

Project description:Dictyostelium discoideum is a professional phagocyte and it uses phagocytosis as a way to get food. However, there are a number of bacteria that subvert this professional phagocyte. In this study we investigated whether Salmonella typhimurium is also pathogenic for D. discoideum.

2010-03-10 | GSE20651 | GEO

Differential Expression upon infection with Salmonella typhimurium (t3)

2010-03-10 | GSE20644 | GEO

Differential Expression upon infection with Salmonella typhimurium (t2)

2010-03-10 | GSE20642 | GEO

Differential Expression upon infection with Salmonella typhimurium (t1)

2010-03-10 | GSE20640 | GEO

Differential Expression upon infection with Salmonella typhimurium (t0)

2010-03-10 | GSE20632 | GEO

SopB and SifA modify the proteome of the Salmonella-containing vacuole in the social amoeba Dictyostelium discoideum

Project description:Salmonella enterica serovar Typhimurium is a rod-shaped Gram-negative bacterium. It is a leading cause of gastroenteritis and public health problem. In the environment, it interacts with protozoa, particular amoeba, however the interactions between Salmonella and these eukaryotic microbes have not been addressed in detail. We and others recently described that S. Typhimurium is able to survive in the model amoeba Dictyostelium discoideum requiring the Salmonella pathogenicity islands 1 and 2 (SPI-1 and SPI-2, respectively)-encoded type three secretion systems (T3SSs). In this work, we investigated the role of two particular effector proteins, SopB and SifA that are secreted by either one of the T3SSs. SopB and SifA are involved in the remodelling of the intracellular compartment that contains Salmonella (Salmonella-containing vacuole, SCV) in other cellular models. We combined genetic and proteomics analysis to investigate the roles of SopB and SifA during infections of D. discoideum. We identified over 1,000 proteins per sample performing proteomics on fractions enriched in SCVs from amoeba cells infected with wild-type, sopB or sifA S. Typhimurium. Among them, we observed several Rho GTPases, guanine nucleotide exchange factors and motor proteins. Finally, we decided to evaluate if the changes observed in the proteome from the SCV of wild-type and mutants affect the intracellular survival of S. Typhimurium. These finding suggest that Salmonella exploits this route to survive intracellularly, a process that requires SopB and/or SifA effectors. To our knowledge this is the first proteomic description of the Salmonella intracellular compartment in D. discoideum.

2020-09-23 | PXD014955 | Pride