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Niche-inspired tubuloid differentiation enables modeling of the distal nephron in health and disease

ABSTRACT: Tubuloid culture allows long-term expansion of primary human renal epithelium. However, the progenitor state in expanding tubuloids comes at expense of differentiation, which limits application. To improve differentiation, we developed niche-inspired culture conditions. A single cell atlas revealed that differentiation predominantly generated ascending limb of Henle and principal cells. Using differentiated tubuloids, we subsequently modeled lithium nephropathy in vitro, and performed single-cell RNA sequencing to look at the transcriptional effects caused by lithium. We found that lithium suppresses electrolyte and solute transporters in multiple cell types and induces a progenitor state in kidney tubuloids.

ORGANISM(S): Homo sapiens

PROVIDER: GSE206538 | GEO | 2022/10/18

REPOSITORIES: GEO

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