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Effect of bergapten gel on IMQ mice back skin

ABSTRACT: We then performed gene expression profiling analysis using data obtained from RNA-seq of 9 skin tissues including 3 per group in vaseline-treated group, imq model group and bergapten-gel-treated imq model group.

ORGANISM(S): Mus musculus

PROVIDER: GSE207059 | GEO | 2023/07/01

REPOSITORIES: GEO

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effect of bergapten gel on IMQ mice back skin

Project description:effect of bergapten gel on IMQ mice back skin

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RNA-seq analysis of IMQ-treated wt and Trex2-/- skin

Project description:TREX2 is a keratinocyte specific 3â-deoxyribonuclease that participates in the maintenance of skin homeostasis upon damage. This transcriptome analysis identified multiple genes and pathways deregulated by TREX2 loss in the IMQ-induced psoriasis-like model in mouse skin. mRNA sequencing of 5 biological replicates of skin from wild-type mice treated with Imiquimod and 6 of Trex2 knockout mice treated with Imiquimod

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Microarray expression data from P50 mouse full thickness back skin of C57BL/6 mice - various durations of IMQ treatment and timepoints.

Project description:Imiquimod (IMQ) is a topical therapeutic immune activator that causes psoriasiform inflammation in mice. To determine if IMQ-induced inflammation and gene expression changes depended on the time of day in which treatment is administered, we performed gene expression profiling of dorsal mouse back skin by microarray after different durations of topical 1% IMQ treatment (control = no treatment, 6 hr, 24 hr, and 5 days of IMQ treatment) at different times of day (ZT01, ZT07, ZT09 = day-time treatment; ZT13 and ZT19 = night-time treatment). We also performed a time course after IMQ treatment by collecting mouse back skin after 0 (no treatment), 1, 2, 4, 6, and 24 hours post-treatment. Lastly, we determined gene expression changes in response to IMQ in mice deleted for the core circadian clock gene, Bmal1, after 0 (no treatment) and 24 hours post-1% IMQ compared to Wt (both treated and collected during the daytime at ZT09). The results of this study are important as they show that IMQ-induced activation of interferon sensitive genes are diurnal in Wt mice after 6 hours and 24 hours but not after 5 consecutive treatments. Furthermore, we find that interferon sensitive genes are induced more robustly in the skin of Bmal1 KO mice after 24 hr IMQ compared to Wt mice. These results are important for further understanding how the circadian clock regulates immune activation in response to the theraputic agent IMQ. In this dataset, we include the expression data obtained from 28 microarray samples, all of which were generated from whole back skin RNA samples pooled from 5-7 mice per sample.

2020-03-30 | GSE142165 | GEO

Influence of rYopM treatment on IMQ-induced psoriasiform dermatitis

Project description:RNA-seq of skin of vehicle-treated, IMQ-treated, IMQ+rYopM-treated and IMQ+rYopM_LRR1-3 treated mice.

2021-08-16 | GSE118803 | GEO

Genome-wide mapping of 5-hmC in epithelial samples from IMQ-treated skin versus control skin in mice.

Project description:Analysis of the tag densities showed lower 5-hmC levels in both CpG islands and genebodies in Imiquimod (IMQ)-treated epidermal samples. We looked further into loci-specific changes of 5-hmC and found a total of 364 differentially hydroxymethylated regions. Within these regions, 321 genes showed significantly lower 5-hmC levels and 67 genes had significantly higher 5-hmC levels in the IMQ-treated epidermis compared to control.

2019-12-15 | GSE138921 | GEO

RNA-seq analysis of IMQ-treated wt and Trex2-/- skin

Project description:TREX2 is a keratinocyte specific 3’-deoxyribonuclease that participates in the maintenance of skin homeostasis upon damage. This transcriptome analysis identified multiple genes and pathways deregulated by TREX2 loss in the IMQ-induced psoriasis-like model in mouse skin.

2016-09-07 | GSE74528 | GEO

Low-input mRNA Sequencing Analysis of Epidermal Langerhans cells (LC) from Murine Imiquimod (IMQ)-Induced Psoriasis-Like Dermatitis

Project description:Purpose: To compare epidermal LC transcriptomes of control group (wild-type IMQ-untreated C57/BL6 mice) and IMQ group (wild-type IMQ-treated C57/BL6 mice). Results: KEGG pathway analysis implied that lesional LCs were highly enriched in several inflammatory signalling pathways, including cytokine-cytokine receptor interactions, Th1, Th2 and Th17 cell differentiation, tumor necrosis factor signalling and IL-17 signalling pathways, complement and coagulation cascades, and NOD-like receptor signalling and PI3K-Akt signalling pathways. Gene set enrichment analysis plots revealed enrichment of cytokine activity, chemoattractant activity, protein lipid complex binding, and low-density lipoprotein particle binding gene sets in LCs from IMQ+ mice compared with IMQ- mice. Conclusions: These results confirmed a proinflammatory role for epidermal LCs in the pathogenesis of psoriasis.

2020-11-28 | GSE162274 | GEO

Expression data from IMQ-induced psoriasis-like skin inflammation in miR-146a-/- and C57BL6J mice

Project description:miR-146a acts as a negative feedback regulator of inflammation. To investigate the role of miR-146a in psoriasis psoriasiform skin inflammation was indeuced in Mir-146a-/- and wild type mice (C57BL6J) by topical applciation of imiquimod (IMQ)-cream (Aldara). Gene expression profiling (Affymetrix) was used to identify transcriptomic changes associated with psoriasis-like skin inflammation in wild type vs. miR-146a -/-mice. A daily topical dose of 31.25 mg of Aldara cream (5% IMQ) was applied on the right ear of miR-146a -/- and C57BL/6 mice on three consecutive days to induce psorisis-like skin inflammation. Mice were sacrificed at day 4. Ear flaps were collected for total RNA extraction and hybridization on Affymatrix GeneTitan plate format Gene ST 2.1 (mouse).

2016-02-19 | E-GEOD-78057 | biostudies-arrayexpress

Single-cell sequencing quantitative analysis of skin tissues of wild-type and Chrna5-/- mice treated with imiquimod

Project description:Purpose: To understand the molecular processes and cell subsets involved in an increase in the psoriatic inflammatory response in Chrna5 signaling compared with WT mice, we performed single-cell sequencing with dorsal skin tissues after IMQ and vaseline treatment. Methods: Skin mRNA profiles of wile-type (WT) and cholinergic receptor nicotinic alpha 5 subunit knockout (Chrna5-/-)mice were generated by deep sequencing. The samples of KO and WT mice after IMQ and vaseline treatment were processed in terms of alignment, barcode assignment, and UMI counting with Cell Ranger, version 3.0.2 (https://github.com/10XGenomics/cellranger) count pipeline. Results: The cell clustering analysis , the heat map analysis , the cell subpopulations of keratinocytes analysis, the gene enrichment analysis and signal pathway analysis were all carried out . The results of these studies indicated that Chrna5 knockout has a significant effect on the proliferation of keratinocytes after IMQ treatment. Further gene analysis and signal pathway analysis showed that Chrna5 knockout down-regulated the JAK/STAT signal pathway, which is a key signal pathway for the occurrence and development of psoriasis. Conclusions: These results revealed the potential role of Chrna5 in the occurrence and development of psoriasis, and provided a basis for it as a potential drug target.

2022-02-28 | GSE193350 | GEO

Effect of GDNF-Gel/HA-Mg nerve conduit on gene expression during peripheral nerve regeneration

Project description:In this study, glial cell line-derived neurotrophic factor (GDNF)- Gelatin methacryloyl (Gel)/hydroxylapatite (HA)-Mg nerve conduit was developed. the conduit was implanted in sciatic nerve defect model in SD rats to explore its role in repairing peripheral nerve defects. Regenerated nerve tissues from GDNF-Gel/HA-Mg group and negative control group were collected for RNA-seq, aiming to explore the potential molecular mechanism of GDNF-Gel/HA-Mg in peripheral nerve regeneration.

2023-12-20 | GSE243282 | GEO

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