Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The microbiome buffers tadpole hosts from heat stress: a hologenomic approach to understand host-microbe interactions under warming

ABSTRACT: We exposed two groups of green frog tadpoles that differed in their microbiome composition to heat stress or control conditions. We subsequently used RNAseq to profile gene expression in their gut to understand how the microbiome impacts host responses to heat.

ORGANISM(S): Lithobates clamitans

PROVIDER: GSE208645 | GEO | 2023/01/02

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

The microbiome buffers tadpole hosts from heat stress: a hologenomic approach to understand host-microbe interactions under warming.

Project description:Phenotypic plasticity is an important strategy that animals employ to respond and adjust to changes in their environment. Plasticity may occur via changes in host gene expression or through functional changes in their microbiomes, which contribute substantially to host physiology. Specifically, the presence and function of host-associated microbes can impact how animals respond to heat stress. We previously demonstrated that 'depleted' tadpoles, with artificially disrupted microbiomes, are less tolerant to heat than 'colonized' tadpoles, with more natural microbiomes. However, the mechanisms behind these effects are unclear. Here, we compared gene expression profiles of the tadpole gut transcriptome, and tadpole gut microbial metagenome, between colonized and depleted tadpoles under cool or warm conditions. Our goal was to identify differences in host and microbial responses to heat between colonized and depleted tadpoles that might explain their observed differences in heat tolerance. We found that depleted tadpoles exhibited a much stronger degree of host gene expression plasticity in response to heat, while the microbiome of colonized tadpoles was significantly more heat sensitive. These patterns indicate that functional changes in the microbiome in response to heat may allow for a dampened host response, ultimately buffering hosts from the deleterious effects of heat stress. We also identified several specific host and microbial pathways that could be contributing to increased thermal tolerance in colonized tadpoles including amino acid metabolism, vitamin biosynthesis and ROS scavenging pathways. Our results demonstrate that the microbiome influences host plasticity and the response of hosts to environmental stressors.

| S-EPMC10086385 | biostudies-literature

The microbiome buffers tadpole hosts from the deleterious effects of heat stress: a hologenomic approach to understand host-microbe interactions under warming conditions.

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-12-30 | GSE208646 | GEO

The microbiome buffers tadpole hosts from heat stress: a hologenomic approach to understand host-microbe interactions under warming

Project description:The microbiome buffers tadpole hosts from heat stress: a hologenomic approach to understand host-microbe interactions under warming

| PRJNA860544 | ENA

The microbiome buffers tadpole hosts from the deleterious effects of heat stress: a hologenomic approach to understand host-microbe interactions under warming conditions.

Project description:The microbiome buffers tadpole hosts from the deleterious effects of heat stress: a hologenomic approach to understand host-microbe interactions under warming conditions.

| PRJNA860534 | ENA

Effects of Acute Exposure to the Non-steroidal Anti-inflammatory Drug Ibuprofen on the Developing North American Bullfrog (Rana catesbeiana) Tadpole

Project description:An anthropogenic chemical contaminant commonly identified in aquatic receiving environments is the non-steroidal anti-inflammatory drug (NSAID), ibuprofen(IBF). While the role of ibuprofen in target organisms is known, there exists a paucity of data on the impact of exposure to non-target wildlife species. In the case of frog species, normal development and environmental fitness involves the actions of the thyroid hormones (THs), particularly at key points in the life cycle. We investigated whether exposure of premetamorphic North American bullfrog (Rana catesbeiana) tadpoles to IBF altered their response to treatment with an exogenous dose of thyroid hormone (T3).

2014-09-23 | GSE53565 | GEO

Effects of Acute Exposure to the Non-steroidal Anti-inflammatory Drug Ibuprofen on the Developing North American Bullfrog (Rana catesbeiana) Tadpole

2014-09-23 | E-GEOD-53565 | biostudies-arrayexpress

Thyroid hormone induced changes in Xenopus laevis tadpole brain

Project description:Premetamorphic Xenopus laevis tadpoles brain ventricle cells respond to thyroid hormone by proliferation and subsequent differentiation. The goal of this experiment is to identify the genes involved in the TH-induced proliferation pathway in tadpole brain and compare it to TH-induced proliferation and differentiation program in tadpole limb. Xenopus tadpoles (NF54) were treated with 1 mM methimazole in 0.1 X MMR solution for 1 week to block the endogenous TH production and reduce the TH present in the system of the tadpole. They were then treated with 100 nM T3 in 1 mM methimazole and 0.1 x MMR for another 24h and 48h or without T3 for 48h (control group). Brains from the tadpoles were dissected at the end of the experiment. Keywords: development or differentiation design,organism part comparison design,reference design,replicate design,time series design

2005-10-04 | GSE3402 | GEO

Thyroid hormone induced resorption of tail in Xenopus laevis tadpole

Project description:Premetamorphic Xenopus laevis tadpole tail respond to thyroid hormone by resorption. The goal of this experiment is to identify the genes involved in the TH-induced resorption tadpole tail and compare it to TH-induced proliferation and differentiation program in tadpole limb and brain. Xenopus tadpoles (NF54) were treated with 100 nM T3 in 0.1 x MMR for another 24h and 48h or without T3 for 48h (control group). NF 61 tadpoles were in 0.1 X MMR till they reached NF stage 62. The tails were dissected after the experiment. Keywords: development or differentiation design,organism part comparison design,reference design,replicate design,time series design

2005-10-04 | GSE3405 | GEO

Green frog tadpole gut microbiome

Project description:Green frog tadpole gut microbiota across temperatures and microbiome treatments targeted loci environmental

| PRJNA732310 | ENA

DNA methylation dynamics underlie metamorphic gene regulation programs in Xenopus tadpole brain

Project description:Changes in DNA methylation in the preoptic area/hypothalamus/thalamus of Xenopus tropicalis tadpoles were analyzed using Methylated DNA Capture sequencing (MethlCap-seq) at four stages of metamorphosis.

2019-10-23 | GSE139267 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data