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Gene expression profiles in gastric cancer cells with TM4SF1-AS1 knockdown

ABSTRACT: To clarify the function of a long noncoding RNA TM4SF1-AS1 in gastric cancer (GC), we carried out a gene expression microarray analysis using GC cell lines HSC45 transfected with a siRNA targeting TM4SF1-AS1 or a control siRNA. Gene ontology analysis revealed that genes associated with interferon signaling and immune response were significantly enriched among downregulated genes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE209814 | GEO | 2023/07/01

REPOSITORIES: GEO

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TM4SF1-AS1 inhibits apoptosis by promoting stress granule formation in cancer cells

Project description:Long noncoding RNAs (lncRNAs) play pivotal roles in tumor development, but little is known about their involvement in the early steps of tumorigenesis. To identify lncRNAs dysregulated in precancerous lesions, we analyzed genome-wide trimethylation of histone H3 lysine 4 (H3K4me3) to screen for transcriptionally active lncRNA genes in the nontumorous gastric mucosa of patients with gastric cancer (GC) and healthy individuals. We found that H3K4me3 at TM4SF1-AS1 is specifically upregulated in GC patients and that expression of TM4SF1-AS1 is prevalently elevated in primary and cultured GC cells. TM4SF1-AS1 contributes to GC cell proliferation in vitro and in vivo, and its oncogenic function is mediated, at least in part, through interaction with Pur-α and YB-1. Chromatin isolation by RNA purification (ChIRP)-mass spectrometry demonstrated that TM4SF1‑AS1 associates with several stress granule (SG)-related proteins, including G3BP2, RACK1 and DDX3. Notably, TM4SF1-AS1 promotes SG formation and inhibits apoptosis in GC cells by sequestering RACK1, an activator of the stress-responsive MAPK pathway, within SGs. TM4SF1‑AS1-induced SG formation and apoptosis inhibition are dependent on Pur-α and YB-1. These findings suggest TM4SF1-AS1 contributes to tumorigenesis by enhancing SG-mediated stress adaptation.

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TUBA1C: a new potential target of LncRNA EGFR-AS1 promotes gastric cancer progression

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Gene expression profiles in gastric cancer cells with TM4SF1-AS1 knockdown

Project description:Gene expression profiles in gastric cancer cells with TM4SF1-AS1 knockdown

| PRJNA862521 | ENA

Identifying target genes for the long noncoding RNA, GATA2-AS1

Project description:We have identified a set of endothelial-enriched long noncoding RNAs (lncRNAs) in another experiment. In this experiment, we aim to identify genes that are regulated by the GATA2-AS1 lncRNA. We have depleted GATA2-AS1 using two distinct siRNA in human umbilical vein endothelial cells (HUVEC)

2022-02-25 | GSE197376 | GEO

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