Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Regulation of cohesin-mediated chromosome structure by PDS5 in mammals

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE209849 | GEO | 2022/10/08

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Regulation of cohesin-mediated chromosome folding by PDS5 in mammals

Project description:Cohesin regulates sister chromatid cohesion but also contributes to chromosome folding by promoting the formation of chromatin loops, a process mediated by loop extrusion. Although PDS5 regulates cohesin dynamics on chromatin, the exact function of PDS5 in cohesin-mediated chromatin looping remains unclear. Two paralogs of PDS5 exist in vertebrates, PDS5A and PDS5B. Here we show that PDS5A and PDS5B co-localize with RAD21 and CTCF at loop anchors. Rapid PDS5A or PDS5B degradation in liver cancer cells using an inducible degron system reduces chromatin loops and increases loop size. RAD21 enrichment at loop anchors is decreased upon depletion of PDS5A or PDS5B. PDS5B loss also reduces CTCF signals at loop anchors and has a stronger effect on loop enlargement compared with PDS5A. Co-depletion of PDS5A and PDS5B reduces RAD21 levels at loop anchors although the amount of cohesin on chromatin is increased. Our study provides insight into how PDS5 proteins regulate cohesin-mediated chromatin looping.

| S-SCDT-EMBOR-2022-54853V1 | biostudies-other

Regulation of cohesin-mediated chromosome structure by PDS5 in mammals [RNA-seq]

Project description:Cohesin regulates sister chromatids cohesion but also contributes to chromosome folding by promoting the formation of chromatin loops, a process proposed to be mediated by loop extrusion. PDS5 plays a crucial role in regulating the cohesin dynamic on chromatin, mainly through WAPL-mediated release activity and the opponent ESCO1/2-sororin dependent pathway. However, the exact function of PDS5 on cohesin-mediated chromatin looping remains ambiguous. Two versions of PDS5 exist in vertebrates, PDS5A and PDS5B. Here, we construct cells for rapid PDS5A or PDS5B degradation in PLC/PRF/5 cell line with the FKBP12F36V-dTAG degron system to perform loss of function studies. Combining Hi-C, ChIP-seq for cohesin regulators, and RNA-seq data, we describe the redundant and discrete roles of PDS5A and PDS5B in three-dimensional genome organization and gene expression.

2022-10-08 | GSE209848 | GEO

Regulation of cohesin-mediated chromosome structure by PDS5 in mammals [Hi-C]

2022-10-08 | GSE209846 | GEO

Regulation of cohesin-mediated chromosome structure by PDS5 in mammals [ChIP-seq]

2022-10-08 | GSE209845 | GEO

Regulation of cohesin-mediated chromosome structure by PDS5 in mammals

Project description:Regulation of cohesin-mediated chromosome structure by PDS5 in mammals

| PRJNA862699 | ENA

Regulation of cohesin-mediated chromosome structure by PDS5 in mammals [ChIP-seq]

Project description:Regulation of cohesin-mediated chromosome structure by PDS5 in mammals [ChIP-seq]

| PRJNA862700 | ENA

Regulation of cohesin-mediated chromosome structure by PDS5 in mammals [Hi-C]

Project description:Regulation of cohesin-mediated chromosome structure by PDS5 in mammals [Hi-C]

| PRJNA862701 | ENA

Regulation of cohesin-mediated chromosome structure by PDS5 in mammals [RNA-seq]

Project description:Regulation of cohesin-mediated chromosome structure by PDS5 in mammals [RNA-seq]

| PRJNA862702 | ENA

Pds5 promotes cohesin acetylation and stable cohesin-chromosome interaction.

Project description:Pds5 and Wpl1 act as anti-establishment factors preventing sister-chromatid cohesion until counteracted in S-phase by the cohesin acetyl-transferase Eso1. However, Pds5 is also required to maintain sister-chromatid cohesion in G2. Here, we show that Pds5 is essential for cohesin acetylation by Eso1 and ensures the maintenance of cohesion by promoting a stable cohesin interaction with replicated chromosomes. The latter requires Eso1 only in the presence of Wapl, indicating that cohesin stabilization relies on Eso1 only to neutralize the anti-establishment activity. We suggest that Eso1 requires Pds5 to counteract anti-establishment. This allows both cohesion establishment and Pds5-dependent stable cohesin binding to chromosomes.

| S-EPMC3388792 | biostudies-other

Long-range chromosome interactions mediated by cohesin shape circadian gene expression

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-04-04 | E-GEOD-68832 | biostudies-arrayexpress