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Novel Transcriptomic Network Dynamics in Response to Divergent Acute Exercise Challenges in Young Adults

ABSTRACT: This study measured the transcriptome-wide response in skeletal muscle and circulating extracellular vesicles (EVs) in young adults randomized to a single exposure of TRAD (n=21, 12M/9F, 22±3y) or HITT (n=19, 11M/8F, 22±2y) exercise. Next-generation sequencing captured small (<200nt), long (>200nt), and circular RNA species in muscle and EVs. In parallel, data streams were analyzed to identify transcripts differentially expressed (|log2FC|>1, FDR≤0.05) immediately (h0, EVs only), h3, and h24 postexercise within and between exercise doses. Strongly-trending responsive transcripts (FDR<0.2) underwent singular value decomposition to summarize the data structure into latent variables, LVs) deconvolving inter-regulatory relationships across transcripts; LVs were compared across time and exercise dose.

ORGANISM(S): Homo sapiens

PROVIDER: GSE209880 | GEO | 2023/03/29

REPOSITORIES: GEO

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