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Localized condensation of the cBAF remodeler is governed by ARID1A/B and is required for transcriptional activation

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE209961 | GEO | 2023/10/01

REPOSITORIES: GEO

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Localized condensation of the cBAF remodeler is governed by ARID1A/B and is required for transcriptional activation [CUT&Tag]

Project description:This study investigates the role of the ARID1A/B subunits of the cBAF complex using in vitro and cell-based models. Specifically, we find that the N-terminal disordered region of ARID1A/B and the DNA binding ARID domain are required for condensation and proper orientation on chromatin respectively.

2023-10-01 | GSE209955 | GEO

Localized condensation of the cBAF remodeler is governed by ARID1A/B and is required for transcriptional activation [RNA-seq]

2023-10-01 | GSE209953 | GEO

Localized condensation of the cBAF remodeler is governed by ARID1A/B and is required for transcriptional activation [CUT&RUN]

Project description:Specifically, we find that the N-terminal disordered region of ARID1A/B and the DNA binding ARID domain are required for condensate formation and chromatin targeting.

2023-10-01 | GSE235698 | GEO

Localized condensation of the cBAF remodeler is governed by ARID1A/B and is required for transcriptional activation [ATAC-seq]

2023-10-01 | GSE209959 | GEO

Localized condensation of the cBAF remodeler is governed by ARID1A/B and is required for transcriptional activation

Project description:Localized condensation of the cBAF remodeler is governed by ARID1A/B and is required for transcriptional activation

| PRJNA863231 | ENA

Localized condensation of the cBAF remodeler is governed by ARID1A/B and is required for transcriptional activation [CUT&Tag]

Project description:Localized condensation of the cBAF remodeler is governed by ARID1A/B and is required for transcriptional activation [CUT&Tag]

| PRJNA863227 | ENA

Localized condensation of the cBAF remodeler is governed by ARID1A/B and is required for transcriptional activation [CUT&RUN]

Project description:Localized condensation of the cBAF remodeler is governed by ARID1A/B and is required for transcriptional activation [CUT&RUN]

| PRJNA986923 | ENA

Localized condensation of the cBAF remodeler is governed by ARID1A/B and is required for transcriptional activation [RNA-seq]

Project description:Localized condensation of the cBAF remodeler is governed by ARID1A/B and is required for transcriptional activation [RNA-seq]

| PRJNA863224 | ENA

Localized condensation of the cBAF remodeler is governed by ARID1A/B and is required for transcriptional activation [ATAC-seq]

Project description:Localized condensation of the cBAF remodeler is governed by ARID1A/B and is required for transcriptional activation [ATAC-seq]

| PRJNA863238 | ENA

The chromatin Remodeler CHD8 is required for activation of progesterone receptor-dependent enhancers.

Project description:While the importance of gene enhancers in transcriptional regulation is well established, the mechanisms and the protein factors that determine enhancers activity have only recently begun to be unravelled. Recent studies have shown that progesterone receptor (PR) binds regions that display typical features of gene enhancers. Here, we show by ChIP-seq experiments that the chromatin remodeler CHD8 mostly binds promoters under proliferation conditions. However, upon progestin stimulation, CHD8 re-localizes to PR enhancers also enriched in p300 and H3K4me1. Consistently, CHD8 depletion severely impairs progestin-dependent gene regulation. CHD8 binding is PR-dependent but independent of the pioneering factor FOXA1. The SWI/SNF chromatin-remodelling complex is required for PR-dependent gene activation. Interestingly, we show that CHD8 interacts with the SWI/SNF complex and that depletion of BRG1 and BRM, the ATPases of SWI/SNF complex, impairs CHD8 recruitment. We also show that CHD8 is not required for H3K27 acetylation, but contributes to increase accessibility of the enhancer to DNaseI. Furthermore, CHD8 was required for RNAPII recruiting to the enhancers and for transcription of enhancer-derived RNAs (eRNAs). Taken together our data demonstrate that CHD8 is involved in late stages of PR enhancers activation.

| S-EPMC4403880 | biostudies-literature

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