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Serum response factor reduces gene expression noise and confers cell state stability (Bulk RNA-Seq)

ABSTRACT: To explore whether SRF controls cell state heterogeneity, SRF KO or wild type (WT) iPSCs cultured in serum containing medium.

ORGANISM(S): Mus musculus

PROVIDER: GSE210072 | GEO | 2023/01/01

REPOSITORIES: GEO

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Serum response factor reduces gene expression noise and confers cell state stability (scRNA-Seq)

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Project description:The objective of this study is to assess the early effects of the Serum Response Factor deletion on the vascular gene expression program. To generate smooth muscle specific SRF knockout, 4 month-old [SRF-flex2neo -SM22CreERT2ki/+] mice (SRFSMKO mice) were injected i.p. with 1mg of tamoxifen for 3 days. All control (SRF-flex2neo littermates) and SRFSMKO mice were systematically injected with tamoxifen to normalize for any effects due to tamoxifen.

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