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Transcriptomic analysis in hippocampus of C57BL/6 and DBA/2 mice

ABSTRACT: Background: Previous studies have shown that significant difference in learning and memory ability existed between C57BL/6 and DBA/2 mouse. Identification of behavioral and genetic differences between these two strains is typically important for neuroscience research, indicating that they can be used to identify candidate genes for learning and memory. Objective: This study was conducted to identify candidate genes for learning and memory using C57BL/6 and DBA/2 mouse. Methods: We conducted a transcriptomic analysis in hippocampus of C57BL/6 and DBA/2 mouse using high throughput RNA deep sequencing (RNA-seq) technology to detect differentially expressed genes (DEGs). Differentially expressed genes were then functionally annotated. Results: We found a total of 31,714 genes from hippocampi of C57BL/6 and DBA/2 mice, which comprised 4,241 to 4,599 known genes with new isoforms and, 4,023 to 4,451 structurally optimized known genes. Among these genes, a total of 458 genes showed different expression in the two strains of mouse. Eight genes were randomly selected to be validated by qRT-PCR, and the expression levels of all genes were consistent with the RNA sequencing. Conclusion: Our data indicated that there were different gene expression patterns between hippocampi of C57BL/6 and DBA/2 mice. The genes associated with learning and memory discovered in the study may enhance the explanation of the behavioral differences between the two mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE210595 | GEO | 2022/08/08

REPOSITORIES: GEO

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