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Hog1 mutant 4 hours exposure to congo red (cell wall chip)

ABSTRACT: S. cerevisiae hog1 mutant strain was grown on YEPD. For Congo Red experiments, yest cells were grown overnight at 24 °C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 °C for 2h. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with Congo Red to a final concentration of 30 µg/ml. Cells were collected at 4hours of growth, frozen at -80 °C and processed for RNA extraction. Keywords: repeat sample

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE2107 | GEO | 2005/03/01

SECONDARY ACCESSION(S): PRJNA91807

REPOSITORIES: GEO

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Hog1 mutant 4 hours exposure to congo red (cell wall chip)

Project description:S. cerevisiae hog1 mutant strain was grown on YEPD. For Congo Red experiments, yest cells were grown overnight at 24 Â°C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 Â°C for 2h. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with Congo Red to a final concentration of 30 Âµg/ml. Cells were collected at 4hours of growth, frozen at -80 Â°C and processed for RNA extraction.

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Wild type 4 hours exposure to congo red (cell wall chip)

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Project description:S. cerevisiae was grown on YEPD. For Congo Red experiments, yeast cells were grown overnight at 24 °C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 °C for 2h 30min. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with Congo Red to a final concentration of 30 μg/ml. At this time cells were collected t=0h. Keywords: repeat sample

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