Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profiles of the egg and synergid cell in rice

ABSTRACT: To reveal comprehensive gene expression profiles and their characteristics of the rice egg and synergid cell, we performed cell type-specific microarray analysis using nonenzymatic cell isolation technique. 40 and 50 genes expressed specifically in the egg cell and synergid cell were identified by cluster analysis using microarray results including that of control samples. Furthermore, we found that the differences in the gene expression profiles of the egg cell and the synergid cell were more extensive than previously realized, indicating that these reproductive cells are specified during the short period encompassing the three sequential mitotic nuclear divisions after generation of the megaspore.

ORGANISM(S): Oryza sativa

PROVIDER: GSE21074 | GEO | 2010/12/01

SECONDARY ACCESSION(S): PRJNA126743

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Gene expression profiles of the egg and synergid cell in rice

Project description:To reveal comprehensive gene expression profiles and their characteristics of the rice egg and synergid cell, we performed cell type-specific microarray analysis using nonenzymatic cell isolation technique. 40 and 50 genes expressed specifically in the egg cell and synergid cell were identified by cluster analysis using microarray results including that of control samples. Furthermore, we found that the differences in the gene expression profiles of the egg cell and the synergid cell were more extensive than previously realized, indicating that these reproductive cells are specified during the short period encompassing the three sequential mitotic nuclear divisions after generation of the megaspore. Gene expression analysis in five samples (egg cell, synergid cell, ovary with or without mannitol treatment, and whole plant) were performed. Three biological replicates were prepared for each sample.

2010-12-01 | E-GEOD-21074 | biostudies-arrayexpress

Oryza sativa

Project description:Gene expression profiles of the egg and synergid cell in rice

| PRJNA126743 | ENA

Genes dominantly expressed in germinating pollen

Project description:In sexual reproduction, a proper communication and cooperation between male and female organs and tissue are essential for male and female gametes to unite. In flowering plants, female sporophytic tissues and gametophyte direct a male pollen tube towards an egg apparatus, which consists of an egg cell and two synergid cells. The cell-cell communication between the pollen tube and the egg apparatus, such as the reception of a signal from the egg apparatus at the pollen tube, makes the tip of pollen tube rapture to release the sperm cell. To isolate male factors involved in the interaction between a pollen tube and an egg apparatus, we focused on receptor-like kinases (RLKs), which are extensively diversified in the flowering plant lineage to comprise a large monophyletic gene family. Approximately 620 members were found in the Arabidopsis thaliana genome. Expression patterns of 558 RLKs were analyzed using an Affymetrix ATH1 microarray of A. thaliana. We focused on two RLKs, ANXUR1 (ANX1) and ANXUR2 (ANX2), and characterized their function. Here we report that pollen tubes of anx1/anx2 ruptured before arriving at the egg apparatus, suggesting that ANX1 and ANX2 are male factors controlling pollen tube behavior with directing rupture at proper timing. Furthermore, ANX1 and ANX2 were the most closely related paralogs to a female factor FERONIA/SIRENE controlling pollen tube behavior expressed in synergid cells. Our finding shows that the coordinated behaviors of female and male reproductive apparatuses are regulated by the sister genes, whose duplication might play a role in the evolution of fertilization system in flowering plants.

2009-08-01 | GSE12316 | GEO

Identification of Xenopus laevis egg extract fraction stimulating somatic DNA replication through LC-MSMS

Project description:In several metazoans the number of active replication origins in embryonic nuclei is higher than in somatic ones, ensuring rapid genome duplication during synchronous embryonic cell divisions. High replication origin density can be restored by somatic nuclear reprogramming. However, mechanisms underlying high replication origin density formation coupled to rapid cell cycles are poorly understood. Here, we characterized a fraction of Xenopus egg extract able to stimulate somatic DNA replication.

2020-03-19 | PXD017383 | Pride

Association of egg mass and egg sex in chicken

Project description:Association of egg mass and egg sex: gene expression analysis from maternal RNA in the germinal disc region of layer hens (Gallus gallus)

2015-01-29 | GSE59041 | GEO

Transcriptomic profiling of egg quality in seabass (Dicentrarchus labrax)

Project description:The aim of the project was to identify differently expressed genes in eggs of European seabass (Dicentrarchus labrax) characterized by different quality. In this way it was expected to identify genes possibly being a molecular indicator of egg quality in this species, which was never studied to date. For the study microarray analysis of over 26 thousand genes in 16 egg batches was performed. Additionally, for each egg batch biological quality was determined, what allowed to compare the gene expression profile with overall egg quality (divided into two groups representing ‘high’ and ‘low’ egg quality). The analysis allowed to identify 39 differently expressed genes between the two groups representing ‘high’ and ‘low’ egg quality. From those genes, expression level of 7 were verified by real-time qPCR which confirmed significant difference in expression in 5 of them.

2016-12-31 | GSE84559 | GEO

Comparative gene expression in synergid cells and antipodal cells at anthesis

Project description:Synergid cells represent the receptor cell type of angiosperm gametophytes that attract and receive the male pollen tube and are implicated in the transfer of the sperm cells to the target egg and central cells prior to double fertilization. The antipodals represent an accessory cell type that typically degenerates after fertilization; in rice it may initially have a short-lived nutritional role prior to endosperm establishment. We examined the transcriptome of these two cell types using Oryza sativa ssp. japonica (cv Katy) using the Affymetrix 57K rice genome GeneChip to provide an overview of genes activated at receptivity in the synergid and antipodal cells and transcript up- and down-regulated at female gametophyte maturity just prior to fertilization. This is part of a larger microarray paroject to examine the global program of gene expression during sexual reproduction in Oryza sativa which serves as a model angiosperm in which male and female gametophytic and embryonic cells may be readily isolated and characterized.

2022-12-31 | GSE56108 | GEO

Escherichia coli strain:poultry egg | isolate:poultry egg

Project description:Escherichia coli strain:poultry egg | isolate:poultry egg Genome sequencing

| PRJNA951541 | ENA

Escherichia coli strain:poultry egg | isolate:poultry egg

Project description:Escherichia coli strain:poultry egg | isolate:poultry egg Genome sequencing

| PRJNA951542 | ENA

Escherichia coli strain:poultry egg | isolate:poultry egg

Project description:Escherichia coli strain:poultry egg | isolate:poultry egg Genome sequencing

| PRJNA951208 | ENA