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Wild type yellow experiment (cell wall chip)

ABSTRACT: S. cerevisiae was grown on YEPD. For yellow experiments, yeast cells were grown overnight at 24 °C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 °C for 6h. At this time cells were collected. Experiment was carried out hybridising the cDNA from the same RNA (from this culture) with both Cy3 and Cy5. Keywords: repeat sample

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE2108 | GEO | 2005/03/01

SECONDARY ACCESSION(S): PRJNA91761

REPOSITORIES: GEO

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Wild type yellow experiment (cell wall chip)

Project description:S. cerevisiae was grown on YEPD. For yellow experiments, yeast cells were grown overnight at 24 Â°C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 Â°C for 6h. At this time cells were collected. Experiment was carried out hybridising the cDNA from the same RNA (from this culture) with both Cy3 and Cy5.

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Project description:S. cerevisiae was grown on YEPD. For Zymolyase experiments, yeast cells were grown overnight at 24 °C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 °C for 2h 30min. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with 5units/ml Zymolyase 100T . Cells were collected at 2 hours of growth, frozen at -80 °C and processed for RNA extraction. Keywords: repeat sample

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Project description:S. cerevisiae hog1 mutant strain was grown on YEPD. For Congo Red experiments, yest cells were grown overnight at 24 °C to an optical density 0.8 - 1 (A600). The culture was refreshed to 0.2 O.D and grown at 24 °C for 2h. Next, culture was divided into two parts. One continues growing under same conditions (non-treated culture) while the other was supplemented with Congo Red to a final concentration of 30 µg/ml. Cells were collected at 4hours of growth, frozen at -80 °C and processed for RNA extraction. Keywords: repeat sample

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