Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Microarrays of macrophages infected with smooth or rough Brucella suis

ABSTRACT: The macrophage-Brucella interaction is critical for the establishment of a chronic Brucella infection. Smooth virulent B. suis strain 1330 (S1330) prevents macrophage cell death. However, rough attenuated B. suis strain VTRS1 induces strong macrophage cell death. To further investigate the mechanism of VTRS1-induced macrophage cell death, microarrays were used to analyze temporal transcriptional responses of murine macrophage-like J774. A1 cells infected with S1330 or VTRS1.

ORGANISM(S): Mus musculus

PROVIDER: GSE21117 | GEO | 2011/06/24

SECONDARY ACCESSION(S): PRJNA126471

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Microarrays of macrophages infected with smooth or rough Brucella suis

Project description:The macrophage-Brucella interaction is critical for the establishment of a chronic Brucella infection. Smooth virulent B. suis strain 1330 (S1330) prevents macrophage cell death. However, rough attenuated B. suis strain VTRS1 induces strong macrophage cell death. To further investigate the mechanism of VTRS1-induced macrophage cell death, microarrays were used to analyze temporal transcriptional responses of murine macrophage-like J774. A1 cells infected with S1330 or VTRS1. Murine J774.A1 macrophages were plated in T75 at 8 x 10^6 cells per flask one day prior to infection, and then infected with B. suis S1330 or VTRS1 at a MOI of 200:1. Total RNAs were isolated by TRIzol and further purified using Qiagen RNeasy Mini Kit (Qiagen, Valencia, CA) at 0, 1, 2, 4, 8, 24, and 48 h post infection. The RNA samples were stored at -80 ºC until an Agilent 2100 BioAnalyzer (Agilent Technologies, Palo Alto, CA) was used to assess the concentrations and quality of RNA samples. Total RNA (20 µg) per sample was used for hybridization with Affymetrix mouse GeneChip 430 2.0 array. Preparation of cDNA, hybridization, quality controls and scanning of the GeneChip 430 2.0 arrays were performed according to the manufacturer's protocol (Affymetrix, Santa Clara, CA) .

2011-06-24 | E-GEOD-21117 | biostudies-arrayexpress

Transcriptome-wide identification of Hfq-associated RNAs in Brucella suis by deep sequencing

Project description:We report the identification of new noncoding RNAs in Brucella suis 1330 and that are associated to the chaperone protein Hfq

2015-11-25 | GSE73621 | GEO

Comparative transcriptome analysis of Brucella suis 1330 wild-type and DregA mutant

Project description:Investigation of whole genome gene expression level changes in a B. suis 1330 regA mutant, compared to the wild-type strain. The two-component system RegBA of Brucella suis plays a central role in the control of respiratory systems adapted to oxygen deficiency. The mutant strain is affected in long-term persistence in vitro (this study) and in chronic infection in vivo (Abdou, E et al. 2013, Infect.Immun. 81: 2053-61). Using an original “in vitro model of persistence”, we compare large-scale transcriptome of the wild-type and ∆regA strains to identify the RegA-regulon potentially involved in the set-up of the persistence state.

2017-06-26 | GSE87538 | GEO

Transcriptome-wide identification of Hfq-associated RNAs in Brucella suis by deep sequencing

Project description:We report the identification of new noncoding RNAs in Brucella suis 1330 and that are associated to the chaperone protein Hfq Coimmunoprecipitation using Flag-tagged Hfq as bait

2015-11-25 | E-GEOD-73621 | biostudies-arrayexpress

Integrated proteomic and transcriptomic analyses reveal the roles of Brucella BI-1 in cell division and membrane homeostasis of Brucella suis S2

Project description:To explore the role of Brucella BI-1 in Brucella suis S2, we constructed the Brucella BI-1 deletion mutant strain and its complementary strain. We then determined the effect of Brucella BI-1 deletion on the physiological characteristics of Brucella suis S2 and revealed them via integrated transcriptomic and proteomic analyses. Brucella BI-1 deletion altered the membrane properties of Brucella suis S2 and decreased its resistance to acidic pH, H2O2, polymyxin B, and lincomycin. Additionally, deleting Brucella BI-1 led to defective growth, cell division, and viability in Brucella suis S2. In conclusion, our results revealed that Brucella BI-1 is a bacterial cytoprotective protein involved in membrane homeostasis, cell division, and stress resistance in Brucella suis S2.

2021-02-17 | GSE160911 | GEO

Microarray analysis of gene expression in rams experimentally infected with a rough virulent strain of Brucella ovis

Project description:This SuperSeries is composed of the following subset Series: GSE35612: Microarray analysis of gene expression in rams experimentally infected with a rough virulent strain of Brucella ovis (acute phase) GSE35613: Microarray analysis of gene expression in rams experimentally infected with a rough virulent strain of Brucella ovis (chronic 1 phase) GSE35614: Microarray analysis of gene expression in rams experimentally infected with a rough virulent strain of Brucella ovis (chronic 2 phase) Refer to individual Series

2012-02-08 | E-GEOD-35615 | biostudies-arrayexpress

Brucella suis 1330

Project description:Brucella suis 1330 Genome sequencing and assembly

| PRJNA243899 | ENA

Brucella suis 1330

Project description:Brucella suis 1330 Genome sequencing and assembly

| PRJNA238006 | ENA

Brucella suis 1330

Project description:Brucella suis 1330 genome sequencing for revision

| PRJNA70695 | ENA

Preliminary characterization of gene expression in European wild boar naturally infected with Brucella suis

Project description:Brucella suis infects macrophages and dendritic cells. Wild boars act as reservoirs and carriers of Brucella suis biovar 2, and there is evidence that wild boar can be the main source of infection for domestic pigs through the venereal route. Transmission through this route could be an important path for disesease dissemination. The result from this study will contribute to the overall understanding of the molecular pathogenic mechanisms involved during Brucella suis infection in European wild boar.

2009-09-29 | GSE17492 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data