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Transcriptome-wide identification of Hfq-associated RNAs in Brucella suis by deep sequencing

ABSTRACT: We report the identification of new noncoding RNAs in Brucella suis 1330 and that are associated to the chaperone protein Hfq

ORGANISM(S): Brucella suis 1330

PROVIDER: GSE73621 | GEO | 2015/11/25

SECONDARY ACCESSION(S): PRJNA297439

REPOSITORIES: GEO

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Transcriptome-wide identification of Hfq-associated RNAs in Brucella suis by deep sequencing

Project description:We report the identification of new noncoding RNAs in Brucella suis 1330 and that are associated to the chaperone protein Hfq Coimmunoprecipitation using Flag-tagged Hfq as bait

2015-11-25 | E-GEOD-73621 | biostudies-arrayexpress

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Comparative transcriptome analysis of Brucella suis 1330 wild-type and DregA mutant

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Microarrays of macrophages infected with smooth or rough Brucella suis

Project description:The macrophage-Brucella interaction is critical for the establishment of a chronic Brucella infection. Smooth virulent B. suis strain 1330 (S1330) prevents macrophage cell death. However, rough attenuated B. suis strain VTRS1 induces strong macrophage cell death. To further investigate the mechanism of VTRS1-induced macrophage cell death, microarrays were used to analyze temporal transcriptional responses of murine macrophage-like J774. A1 cells infected with S1330 or VTRS1. Murine J774.A1 macrophages were plated in T75 at 8 x 10^6 cells per flask one day prior to infection, and then infected with B. suis S1330 or VTRS1 at a MOI of 200:1. Total RNAs were isolated by TRIzol and further purified using Qiagen RNeasy Mini Kit (Qiagen, Valencia, CA) at 0, 1, 2, 4, 8, 24, and 48 h post infection. The RNA samples were stored at -80 ºC until an Agilent 2100 BioAnalyzer (Agilent Technologies, Palo Alto, CA) was used to assess the concentrations and quality of RNA samples. Total RNA (20 µg) per sample was used for hybridization with Affymetrix mouse GeneChip 430 2.0 array. Preparation of cDNA, hybridization, quality controls and scanning of the GeneChip 430 2.0 arrays were performed according to the manufacturer's protocol (Affymetrix, Santa Clara, CA) .

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Brucella suis 1330

Project description:Brucella suis 1330 Genome sequencing and assembly

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Transcription profiling of European wild boar naturally infected with Brucella suis

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