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Transcriptomic comparison of fructose and lipid grown cultures of Ralstonia eutropha H16

ABSTRACT: Whole genome gene expression analysis was examined with Ralstonia eutropha strain H16 cultures grown in PHB production medium (recipe per Peoples and Sinskey, 1989) containing fructose or trioleate as the main carbon source. The goal of this analysis was to determine the identity of the triacylglycerol and fatty acid breakdown genes in R. eutropha strain H16.

ORGANISM(S): Cupriavidus necator Cupriavidus necator H16

PROVIDER: GSE21145 | GEO | 2010/08/27

SECONDARY ACCESSION(S): PRJNA126669

REPOSITORIES: GEO

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Transcriptomic comparison of fructose and lipid grown cultures of Ralstonia eutropha H16

Project description:Whole genome gene expression analysis was examined with Ralstonia eutropha strain H16 cultures grown in PHB production medium (recipe per Peoples and Sinskey, 1989) containing fructose or trioleate as the main carbon source. The goal of this analysis was to determine the identity of the triacylglycerol and fatty acid breakdown genes in R. eutropha strain H16. In the study presented here, triplicates of R. eutropha strain H16 were examined for changes in expression of 6702 genes during growth and PHB production on each carbon source.

2010-08-26 | E-GEOD-21145 | biostudies-arrayexpress

RNA-seq analysis of polyhydroxyalkanoate-producing Ralstonia eutropha H16

Project description:Ralstonia eutropha H16 is well-known to produce poly(3-hydroxybutyrate) [P(3HB)], a kind of polyhydroxyalkanoates attracted as bio-based biodegradable plastics, efficiently as an energy storage material under unbalanced growth conditions. To obtain further extended knowledge of PHA biosynthesis, this study employed quantitative transcriptome analysis based on deep sequencing of complementary DNA generated from RNA (RNA-seq) for R. eutropha H16. Total RNAs were extracted from R. eutropha cells in growth, PHA production, and stationary phases on fructose. rRNAs in the preparation were removed by repeated treatments with magnetic beads specific to bacterial rRNAs, and then the 36 bp sequences were determined by using Illumina high-throughput sequencer. The RNA-seq results supported induction of gene expression for transcription, translation, cell division, peptidoglycan biosynthesis, pilus and flagella assembly, energy conservation, and fatty acid biosynthesis in growth phase, and repression trends for genes involved in central metabolisms in PHA production phase. Interestingly, transcription of genes for Calvin-Benson-Bassham (CBB) cycle and several selected genes for ?-oxidation were significantly induced in PHA production phase even when the cells were grown on fructose. mRNA profiles of R. eutropha H16 grown on fructose at different phases were generated by deep sequencing, in duplicate, using Illumina GAIIx.

2014-06-07 | E-GEOD-47759 | biostudies-arrayexpress

RNA-seq analysis of polyhydroxyalkanoate-producing Ralstonia eutropha H16

Project description:Ralstonia eutropha H16 is well-known to produce poly(3-hydroxybutyrate) [P(3HB)], a kind of polyhydroxyalkanoates attracted as bio-based biodegradable plastics, efficiently as an energy storage material under unbalanced growth conditions. To obtain further extended knowledge of PHA biosynthesis, this study employed quantitative transcriptome analysis based on deep sequencing of complementary DNA generated from RNA (RNA-seq) for R. eutropha H16. Total RNAs were extracted from R. eutropha cells in growth, PHA production, and stationary phases on fructose. rRNAs in the preparation were removed by repeated treatments with magnetic beads specific to bacterial rRNAs, and then the 36 bp sequences were determined by using Illumina high-throughput sequencer. The RNA-seq results supported induction of gene expression for transcription, translation, cell division, peptidoglycan biosynthesis, pilus and flagella assembly, energy conservation, and fatty acid biosynthesis in growth phase, and repression trends for genes involved in central metabolisms in PHA production phase. Interestingly, transcription of genes for Calvin-Benson-Bassham (CBB) cycle and several selected genes for β-oxidation were significantly induced in PHA production phase even when the cells were grown on fructose.

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Transcriptomic comparison of fructose and lipid grown cultures of Ralstonia eutropha H16

Project description:Transcriptomic comparison of fructose and lipid grown cultures of Ralstonia eutropha H16

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Transcription profiling by array of Ralsonia eutropha H16 wild type and glucose-utilizing mutant G+1 cultivated in medium with fructose or glucose

Project description:In order to identify genes which contribute on the uptake of glucose into cells of the mutant R. eutropha G+1, a genome wide transcription analyses was done. Transcripts of strain H16 and the glucose-utilizing mutant R. eutropha G+1, cultivated in mineral salts medium supplemented with either fructose or glucose were compared.

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Anoxia in Ralstonia eutropha H16

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ralstonia infection-characterisation of wat1 mutant

Project description:rs08-04_wat1-ralstonia - ralstonia infection - identification of the role of the plant cell wall in the interactions between plants and pathogenic agents - Comparison between the mutant and the wt at different time after infection with ralstonia bacteria Keywords: treated vs untreated comparison

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Ralstonia solanacearum_GMI1000 and UW551_20 C and 28 C.

Project description:This SuperSeries is composed of the following subset Series: GSE33657: Expression analysis of Ralstonia solanacearum strain GMI1000 at 20°C and 28°C in rich medium (CPG) and in planta GSE33661: Expression analysis of Ralstonia solanacearum strain UW551 at 20°C and 28°C in rich medium (CPG) and in planta Refer to individual Series

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Project description:ra08-02_clavata-ralstonia - clavata-ralstonia - Clavata1 (Clv1.12) and clavata2 (RPL10) mutants display an increased resistance to the bacteria Ralstonia solanacearum. Transcriptome analysis aims to understand resistance mechanisms. - 3 biological repets Keywords: wt vs mutant comparison

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