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Nanopore RNA sequencing in S. cerevisiae strains defective for decapping and deadenylation

ABSTRACT: RNA turnover in eukaryotes is triggered by decapping, believed to be activated mostly after deadenylation. To investigate simultaneous changes in RNA levels and poly(A) tail size we performed Nanopore RNA sequencing on total RNA extracted from S. cerevisiae strains in which the decapping enzyme Dcp2, or the deadenylation factors Ccr4 and Pop2 were depleted through an inducible degron system. Two fully independent replicate experiments were performed for wild type and the two mutant conditions.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE211782 | GEO | 2022/10/01

REPOSITORIES: GEO

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Nanopore RNA sequencing in S. cerevisiae strains defective for decapping and deadenylation

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