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Differential expression analysis of T. thermophilus HB8 wild-type strain in response to iron

ABSTRACT: We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 0 and 30 min after the addition of FeSO4.

ORGANISM(S): Thermus thermophilus HB8

PROVIDER: GSE21199 | GEO | 2010/10/15

SECONDARY ACCESSION(S): PRJNA129457

REPOSITORIES: GEO

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Differential expression analysis of T. thermophilus HB8 TTHA0995-deficient strain in response to iron

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T. thermophilus HB8 wild-type strain in response to iron (2008)

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Differential expression analysis of T. thermophilus HB8 wild-type strain in response to iron

Project description:We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 0 and 30 min after the addition of FeSO4. Three wild-type strain of T. thermophilus HB8 were pre-cultured at 70 degC for 16 hours in 4 mL of synthetic medium. The cells (2 mL) were inoculated into 200 mL of the same medium and then cultivated at 70Â°C for 9 hours (A600 value being ~0.31). Then the 1.0 mM of FeSO4 was added into the medium, and continued cultivation. Cells were collected at 0 and 30 min time point after the addition of iron ion, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample.

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