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T. thermophilus HB8 wild-type strain in response to iron (2008)

ABSTRACT: We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 30 min after the addition of FeSO4.

ORGANISM(S): Thermus thermophilus HB8

PROVIDER: GSE21466 | GEO | 2010/10/15

SECONDARY ACCESSION(S): PRJNA129539

REPOSITORIES: GEO

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Differential expression analysis of T. thermophilus HB8 wild-type strain in response to iron

Project description:We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 0 and 30 min after the addition of FeSO4.

2010-10-15 | GSE21199 | GEO

Differential expression analysis of T. thermophilus HB8 TTHA0995-deficient strain in response to iron

Project description:We observed the expression profile of the total mRNA in Thermus thermophilus HB8 TTHA0995 deficient strain at 0 and 30 min after the addition of FeSO4.

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T. thermophilus HB8 wild-type strain in response to iron (2008)

Project description:We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 30 min after the addition of FeSO4. Three wild-type strain of T. thermophilus HB8 were pre-cultured at 70 degC for 4 hours in 5 mL of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells diluted 100 fold by the synthetic medium and then cultivated at 70Â°C until A600 value being 1.0 . Then the 1.0 mM of FeSO4 was added into the medium, and continued cultivation. Cells were collected at 0 and 30 min time point after the addition of iron ion, and then crude RNA was extracted. The expression of each mRNA was analyzed on a GeneChip as described under Sample Description Sheet of each sample.

2010-10-15 | E-GEOD-21466 | biostudies-arrayexpress

Differential expression analysis of T. thermophilus HB8 wild-type strain in response to iron

Project description:We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 0 and 30 min after the addition of FeSO4. Three wild-type strain of T. thermophilus HB8 were pre-cultured at 70 degC for 16 hours in 4 mL of synthetic medium. The cells (2 mL) were inoculated into 200 mL of the same medium and then cultivated at 70Â°C for 9 hours (A600 value being ~0.31). Then the 1.0 mM of FeSO4 was added into the medium, and continued cultivation. Cells were collected at 0 and 30 min time point after the addition of iron ion, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample.

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Differential expression analysis of T. thermophilus HB8 TTHA0995-deficient strain in response to iron

Project description:We observed the expression profile of the total mRNA in Thermus thermophilus HB8 TTHA0995 deficient strain at 0 and 30 min after the addition of FeSO4. Three TTHA0995 deficient strain of T. thermophilus HB8 were pre-cultured at 70 degC for 16 hours in 3 mL of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 mL) were inoculated into 1 L of the same medium and then cultivated at 70Â°C for 5.5 hours (A600 value being ~1.53). Then the 1.0 mM of FeSO4 was added into the medium, and continued cultivation. Cells were collected at 0 and 30 min time point after the addition of iron ion, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample.

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Project description:We observed the expression profile of the total mRNA in Thermus thermophilus HB8 csrR deficient strain at 0 and 30 min after the addition of CuSO4.

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Differential expression analysis of T. thermophilus HB8 wild-type strain in response to copper.

Project description:We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 0 and 30 min after the addition of CuSO4.

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