Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-wide maps of Argonaute 2-chromosome interactions in miR-204-3p-overexpressed macrophages [ChIP-Seq]

ABSTRACT: Ago2, an essential effector for miRNA function, has been reported to be found in the nucleus together with miRNA. The miRNA-Ago complexes in the nucleus play an important role in regulating gene transcription. We previously have demonstrated the nuclear presence of miR-204-3p in macrophages. Here, we investigated and compared the Ago2 occupancy on the chromosomes upon miR-204-3p overexpression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE212935 | GEO | 2022/09/13

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptomic analysis of miR-204-3p-overexpressed human monocyte-derived macrophages (HMDMs)

Project description:HMDMs were transfected with miRNA mimics negative control or miRNA-204-3p mimics for 24 hours, and then were sequenced to determine gene expression profiles.

2021-02-03 | GSE166049 | GEO

RNA-seq analysis of mouse bone marrow-derived macrophages overexpressing miR-204-3p

Project description:Bone marrow-derived macrophages were transfected with miRNA-204-3p mimics or mimics negative control for 24 hours. RNA-seq was performed to compare miR-204-3p overexpression versus control cells.

2023-03-01 | GSE223129 | GEO

Genome-wide maps of chromatin state changes in miR-204-3p-overexpressed human monocyte-derived macrophages (HMDMs)

Project description:We compared histone modification states between miRNA-204-3p and mimics negative control transfected HMDMs through ChIP. sequencing

2020-12-04 | GSE162599 | GEO

Impaired AGO2/miR-185-3p/NRP1 Axis Promotes Colorectal Cancer Metastasis

Project description:Increasing evidence suggests that global downregulation of miRNA expression is a hallmark of human cancer, potentially due to defects in the miRNA processing machinery. In this study, we found that the protein expression of Argonaute 2 (AGO2), a key regulator of miRNA processing, was downregulated in colorectal cancer (CRC) tissues, which was also consistent with the findings of the Clinical Proteomic Tumor Analysis Consortium (CPTAC). Furthermore, the correlation between the levels of AGO2 and epithelial-mesenchymal transition (EMT) markers (E-cadherin and vimentin) indicated that reduced levels of AGO2 promoted EMT in CRC. Low expression of AGO2 was an indicator of a poor prognosis among CRC patients. Knockdown of AGO2 in CRC cells promoted migration, invasion and metastasis formation in vitro and in vivo but had no influence on proliferation. To provide detailed insight into the regulatory roles of AGO2, we performed integrated transcriptomic, quantitative proteomic and microRNA sequencing (miRNA-seq) analyses of AGO2 knockdown cells and the corresponding wild-type cells and identified neuropilin 1 (NRP1) as a new substrate of AGO2 via miR-185-3p. Our data provided evidence that knockdown of AGO2 resulted in a reduction of miR-185-3p expression, leading to the upregulation of the expression of NRP1, which is a direct target of miR-185-3p, and elevated CRC cell metastatic capacity. Inhibition of NRP1 or treatment with a miR-185-3p mimic successfully rescued the phenotypes of impaired AGO2, which suggested that therapeutically targeting the AGO2/miR-185-3p/NRP1 axis may be a potential treatment approach for CRC.

2021-02-11 | PXD024171 |

MiR-204-3p inhibits the production of TLR4-related cytokines in familial Mediterranean fever by targeting the PI3Kγ signaling pathway

Project description:Our data suggest that serum miR-204-3p has a potential as a useful biomarker among patients with FMF and that miR-204-3p plays a critical role as a suppressor to regulate the production of TLR4-related cytokines by targeting PI3K signaling pathway.

2017-06-01 | GSE98940 | GEO

Transcriptomic analysis of miR-204-3p-overexpressed human monocyte-derived macrophages (HMDMs)

Project description:Transcriptomic analysis of miR-204-3p-overexpressed human monocyte-derived macrophages (HMDMs)

| PRJNA699045 | ENA

Genome-wide maps of Argonaute 2-chromosome interactions in miR-204-3p-overexpressed macrophages [ChIP-Seq]

Project description:Genome-wide maps of Argonaute 2-chromosome interactions in miR-204-3p-overexpressed macrophages [ChIP-Seq]

| PRJNA878491 | ENA

MicroRNA-24-3p is overexpressed in Hodgkin lymphoma and protects Hodgkin and Reed-Sternberg cells from apoptosis [ChIP-chip]

Project description:Micro (mi)RNAs not only play important roles in biological processes such as proliferation, metabolism, differentiation and apoptosis, but also in diseases such as cancers. We identified miRNAs with deregulated expression in Hodgkin lymphoma (HL) and investigated their role in the pathogenesis of HL. Small RNA sequencing revealed 84 significantly differentially expressed miRNAs in HL cell lines as compared to GC-B cells. Three upregulated miRNAs, miR-23a-3p, miR-24-3p and miR-27a-3p, were derived from one primary-miRNA transcript. Loss-of-function analysis for these miRNAs and their seed family members resulted in decreased growth upon miR-24-3p inhibition in three and of miR-27a/b-3p inhibition in one HL cell line. Apoptosis analysis indicated that the effect of miR-24-3p on cell growth is at least in part caused by an increase of apoptotic cells. Argonaute 2 (Ago2)-IP revealed 1,142 genes consistently targeted by miRNAs in at least three out of four HL cell lines. Furthermore, 52 out of the 1,142 genes were predicated targets of miR-24-3p. Functional annotation analysis revealed a function related to cell growth, cell death and/or apoptosis for 15 out of the 52 genes. Western blotting of the top-5 genes showed increased protein levels upon miR-24-3p inhibition for CDKN1B and MYC. In summary, we showed that miR-24-3p is upregulated in HL and its inhibition impaired cell growth possibly via targeting CDKN1B and MYC.

2017-02-26 | GSE92615 | GEO

MicroRNA-24-3p is overexpressed in Hodgkin lymphoma and protects Hodgkin and Reed-Sternberg cells from apoptosis [small RNA-Seq]

2017-02-26 | GSE92616 | GEO

Determining miR-221/222-3p targets in B cells activated with aCD40 IL4 IL21 in vitro

Project description:compared miR-221/222-3p deficient (KO) and WT (Flox) activated B cell mRNAseq and Ago2-HITSCLIP to determine mRNA targets of this miRNA cluster

2021-01-01 | GSE153811 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data