Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Neonatal necrotizing enterocolitis-associated DNA methylation signatures in the colon are evident in stool samples of affected individuals

ABSTRACT: Neonatal necrotizing enterocolitis (NEC) is a deadly and unpredictable gastrointestinal disease, for which no biomarkers exist. We aimed to describe the methylation patterns in stool and colon from infants with NEC.

ORGANISM(S): Homo sapiens

PROVIDER: GSE212997 | GEO | 2022/09/13

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Selective hypermethylation is evident in small intestine samples from infants with necrotizing enterocolitis [targeted_ileum]

Project description:Necrotizing enterocolitis (NEC) is the most common and lethal gastrointestinal disease affecting preterm infants. NEC develops suddenly and is characterized by gut barrier destruction, an inflammatory response, intestinal necrosis and multi-system organ failure. There is currently no method for early NEC detection, and the pathogenesis of NEC remains unclear.

2022-09-13 | GSE212913 | GEO

Global hypermethylation of intestinal epithelial cells is a hallmark feature of neonatal surgical necrotizing enterocolitis [lcm_colon_ileum]

Project description:Necrotizing enterocolitis (NEC) remains one of the overall leading causes of dealth in premature infants, and the pathogenesis is unpredictable and not well characterized. The aim of our study was to determine the molecular phenotype of NEC via transcriptomic and epithelial cell-specific epigenomic analysis, with a specific focus on DNA methylation.

2022-09-20 | GSE213532 | GEO

Amniotic fluid and necrotizing enterocolitis in preterm pigs

Project description:Preterm neonates are susceptible to gastrointestinal (GI) disorders such as necrotizing enterocolitis (NEC). Maternal milk, and especially colostrum, protects against NEC via growth promoting, immunomodulatory and antimicrobial factors. The fetal enteral diet, amniotic fluid (AF), contains similar bioactive components and we hypothesized that postnatal AF administration would reduce inflammatory responses and NEC in preterm neonates. Thirty preterm pigs (92% gestation) were delivered by caesarean section and fed total parental nutrition (TPN) for 48 h followed by enteral porcine colostrum (COLOS, n=7), infant formula (FORM, n=13) or formula + porcine AF (AF, n=10). Using a previously validated model of NEC in preterm pigs, we determined the structural, functional, microbiological and immunological responses to AF when administered prior to and after introduction of a suboptimal enteral formula diet. Keywords: Healthy versus inflammed tissues in relation to necrotizing enterocolitis Pigs from each treatment group (COLOS, n=4; FORM, n=6; and AF, n=7) were randomly selected for microarray analysis of frozen distal small intestine samples. The FORM group was further divided into formula-fed healthy pigs (F-HEA, n=3) and formula-fed NEC pigs (F-NEC, n=3) in order to compare sick versus healthy formula fed pigs. Equal amounts of total distal small intestinal RNA from all pigs were pooled to make the reference sample. Samples and reference pool were labelled with Oyster 550 and 650, respectively. The in-house spotted porcine oligonucleotide microarray version 4 (POM4) is a low density microarray consisting of 384 different oligonucleotide probes representing more than 200 different immune related genes.

2010-05-06 | E-GEOD-13515 | biostudies-arrayexpress

Amniotic fluid and necrotizing enterocolitis in preterm pigs

2010-03-01 | GSE13515 | GEO

Effect of formula feeding and hypoxia on Necrotizing Enterocolitis (NEC) development

Project description:Necrotizing Enterocolitis (NEC) is an inflammation causing injury to the bowel in newborns. This project uses a rodent model that mimics the intestinal pathological changes seen in NEC to study the effect of formula feeding and hypoxia on NEC development Keywords: time series, diet, hypoxia

2003-07-16 | GSE491 | GEO

Transcription profiling time series of the effects of hypoxia, breast and formula feeding on a Rattus norvegicus model of necrotizing enterocolitis

2007-10-11 | E-GEOD-491 | biostudies-arrayexpress

Temporal development of necrotizing enterocolitis in the preterm pig

Project description:Necrotizing enterocolitis (NEC), a serious gastrointestinal disease that afflicts 5-10% of preterm infants, often progresses rapidly from mild food intolerance into extensive haemorrhage, inflammation and necrosis. Events leading to NEC have remained poorly defined. Similar disease characteristics are observed in preterm pigs 24-48 h after feeding formula. Using this model, we aimed to characterize the temporal development of NEC, and describe the functional and immunological response of the preterm intestine preceding NEC. Keywords: time course

2010-03-01 | GSE13516 | GEO

Microfluidic Device Facilitates In Vitro Modeling of Human Neonatal Necrotizing Enterocolitis-on-a-Chip

Project description:Necrotizing enterocolitis is an intestinal disease induces rapid destruction of the epithelial monolayer of the neonatal intestine. Clinically relevant models of this disease are lacking. Using our novel microfluidic model of necrotizing enterocolitis, we characterized the response of intestinal epithelial cell-derived organoids to intestinal bacteria isolated from a neonate with fatal NEC. We subsequently used RNA-sequencing to determine the gene expression profile of these cells after 24 or 72 hours of incubation.

2023-06-14 | GSE226086 | GEO

Spatial Transcriptomics and Network-Based Bioinformatics Differentiate Intestinal Phenotypes of Cardiac and Classical Necrotizing Enterocolitis

Project description:Classical necrotizing enterocolitis (classical NEC) is a devastating gastrointestinal disease of the neonatal period, and cardiac NEC is a subset of the disease occurring in infants with comorbid congenital heart disease. Despite nearly identical symptomatology, the two NEC subtypes may represent etiologically distinct diseases. We used spatial transcriptomics and network analyses to compare tissue regions of interest derived from surgically resected ileum of patients with cardiac and classical NEC, finding evidence classical and cardiac NEC may arise from differing etiologies.

2024-11-28 | GSE268423 | GEO

Git2 deficiency promotes MDSCs recruitment in intestine and ameliorates necrotizing enterocolitis.

Project description:Necrotizing enterocolitis (NEC) is a life-threatening gastrointestinal disease in newborns. Myeloid-derived suppressor cells (MDSCs) play an important role in immunosuppressive function and ameliorate NEC. G protein-coupled receptor kinase-interacting protein 2 (GIT2) has been implicated in multiple immune processes. Our study demonstrates that Git2 deficiency in mice can promote MDSCs recruiment in intestine. This data will reveal that the mechanism of MDSCs' intestinal gathering in Git2-knockout mice compares to wilde type mice.

2024-10-01 | GSE246144 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data