Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The role of a mammalian network in regulating ischemic stroke outcome III

ABSTRACT: To detect circRNAs, lncRNAs and mRNAs in oxygen and glucose deprivation (OGD) conditions we cultured cortical neurons Embryonic day 15 and subjected them to OGD We performed differentially expression analysis of RNA-seq of wild-type and ciRS-7 KO neurons

ORGANISM(S): Mus musculus

PROVIDER: GSE213179 | GEO | 2023/01/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

The role of a mammalian network in regulating ischemic stroke outcome I

Project description:To detect miRNAs in oxygen and glucose deprivation (OGD) conditions we cultured cortical neurons Embryonic day 15 and subjected them to OGD We performed differentially expression analysis of miRNA-seq of wild-type and ciRS-7 KO neurons

2023-01-31 | GSE213067 | GEO

The role of a mammalian network in regulating ischemic stroke outcome II

Project description:To detect mRNAs in ischemic stroke animals we dissected contralateral (CL) and peri-ischemic (PI) cortex of transient middle cerebral artery occlusion (tMCAo) mice wild-type and ciRS-7 KO We performed differentially expression analysis of RNA-seq of wild-type and ciRS-7 KO tMCAo ischemic mice

2023-01-31 | GSE213177 | GEO

miRNA profiling of control and ischemic samples in vitro and in vivo

Project description:miRNA microarray profiling of primary cortical neurons exposed to 4h oxygen and glucose deprivation (OGD) in vitro. RNA samples collected at 8h post-OGD terminaion. miRNAs were also profiled in mice exposed to 3- vessel occlusion model of stroke. RNA samples collected from contralateral (control) and ipsilateral (infarct) sides of the brain following 24h of reperfusion. Comparison of control and ischemic samples across 3 biological replicates for both in vitro and in vivo samples. In vitro cultures consisted of primary cortical neurons derived from E17 rat Wistar embryos. In vivo samples were obtained from C57bl/6 mice exposed to three-vessel occlusion.

2013-11-21 | E-GEOD-51586 | biostudies-arrayexpress

miRNA profiling of control and ischemic samples in vitro and in vivo

2013-11-21 | GSE51586 | GEO

AGO CLIP reveals an activated network for acute regulation of brain glutamate homeostasis in ischemic stroke [dataset 5]

Project description:Cortical cultures treated with OGD (oxygen glucose deprivation) in the presence of miR-29 expressing lenti-virus were analyzed by RNAseq to assess differences in miR-29 target gene expression.

2019-06-14 | GSE104047 | GEO

Effects of extracellular vehicles secreted from human-induced pluripotent stem cell-derived mesenchymal stem cells on the functional recovery of an ischemic stroke mouse model

Project description:To investigate the the expression profile and potential pathways involved in celluar ischemic stroke model, we established murine HT22 cell lines with oxygen-glucose deprivation/reoxygenation (OGD/R) in vitro

2022-12-31 | GSE208008 | GEO

SUMOylation promotes survival and integration of neural stem cell grafts in stroke

Project description:In this study we sought to determine the effect of overexpressing the SUMOylation E2 conjugase Ubc9 on the response of murine Neural Stem Cells (NSCs) to oxygen-glucose-deprivation and restoration of oxygen/glucose (OGD/ROG). We established stably-expandable lines of NSCs from the subventricular zones (SVZ) of adult wild-type mice (WT NSCs) and Ubc9-overexpressing mice (Ubc9 NSCs) and profiled their transcriptional changes in response to OGD/ROG as well as in response to differentiation.

2019-04-11 | E-MTAB-7428 | biostudies-arrayexpress

The role of a mammalian network in regulating ischemic stroke outcome III

Project description:The role of a mammalian network in regulating ischemic stroke outcome III

| PRJNA879453 | ENA

Long noncoding RNA expression profiles in brain ischemic tolerance by cerebral ischemic preconditioning

Project description:In this study, cerebral cortical astrocytes and neurons of rats were co-cultured, the astrocytes of the co-cultured cell model pretreated by ischemic preconditioning (45 min of OGD) were subjected to lncRNA high-throughput sequencing (RNA-seq).

2021-11-01 | GSE185931 | GEO

In vitro ischemia triggers a transcriptional response to down-regulate synaptic proteins in hippocampal neurons.

Project description:The present study has used whole-rat genome microarray expression profiling to identify genes whose expression is significantly altered in hippocampal neuronal cultures submitted to oxygen and glucose deprivation (OGD), an established in vitro model for cerebral global ischemia that is suitable for investigations at the molecular level. To do so, total RNA was extracted from hippocampal neuronal cultures at an early (7h) and delayed (24h) time point after OGD, as well as from control neurons. Analysis of gene ontology showed that OGD followed by 7h or 24h of recovery induces changes in the expression levels of genes related with inflammation, response to oxidative stress, metabolism, apoptosis, synaptic proteins and ion channels and, importantly, genes that show different expression levels are mainly specific to one of the two time points of recovery analyzed. The expression levels of several genes were confirmed by qPCR and were in good agreement with the microarray data, showing that the combined use of the OGD model and the microarray technology can be a useful tool for the study molecular mechanisms contributing to the neuronal demise after transient global ischemia. Ischemia induced gene expression in primary hippocampal neuronal rat cultures was measured at 7 and 24 hours after exposure to Oxygen and Glucose deprivation (OGD). Three independent experiments were performed at each time (7 or 24 hours) as independent biological replicates.

2014-06-16 | E-GEOD-54037 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data