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Drought induced transcriptomic changes in tobacco

ABSTRACT: The drought stress is one of key adverse environmental factors limiting plant growth and development, even threating global crop productivity in many arid and semi-arid regions. Drought stress usually causes huge economic losses for tobacco industries. Understanding how plants respond and adapt to the drought stress helps generate engineered plants with enhanced drought resistance. In this study, integrative analyses of multiple time point-related transcriptome and metabolome generated from K326 and its derived mutant 28 (M28) with contrasting drought tolerance. We found that dramatic changes of gene expression profiles between M28 and K326 before and after drought treatment.

ORGANISM(S): Nicotiana tabacum

PROVIDER: GSE214048 | GEO | 2022/09/26

REPOSITORIES: GEO

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Project description:Drought stress response is a complex trait regulated at multiple levels. In the past few years, molecular and genomic studies have shown that several drought responsive genes (DRGs) with various functions are induced by drought stresses, and that various transcription factors (TFs) are involved in the regulation of stress-inducible genes. In addition to those DRGs mentioned above, microRNAs (miRNAs) are important regulators of gene expression at the posttranscriptional level by repressing mRNA expression. There is a complex interplay between transcriptional and post-transcriptional regulation of drought response that has not been extensively characterized in tobacco. In order to fully understand DRGs (including TFs) and different roles of miRNAs involved in the stress response, we sequenced and analysed three Digital Gene Expression (DGE) libraries in roots from drought treated tobacco plants, and four small RNA populations in roots, stems and leaves from control or drought treated tobacco plants. We identified 276 candidate DRGs in tobacco with sequence similarities to 64 known DRGs from model plants and crops and about 40% were TFs including WRKY, NAC, ERF and bZIP families. Furthermore, Out of these tobacco DRGs, 54differentially expressed DRGs included 21 TFs, which belonged to 24 TFs families such as NAC (6), MYB (4), ERF (10) and bZIP (1). Additionally, we confirmed expression of 39 known miRNA families (122 members) and five conserved miRNA families, which showed differential regulation under drought stress. Targets of miRNAs were further surveyed based on a recently published study, in which ten targets were DRGs. Finally, an integrated gene regulatory network has been proposed for the molecular mechanisms of the response of tobacco roots to drought stress using differentially expressed DRGs, the changed expression proﬁles of miRNAs and their target transcripts as a basis base on previous studies. In general, our data provide valuable information for future studies of the molecular mechanisms underlying tobacco roots in response to drought resistance in tobacco and other plants.

Project description:Cotton (Gossypium hirsutum L.) is one of the most important cash crops worldwide. In semi-arid/arid regions, drought stress causes growth limitation and decrease of yield. Of all the organs of a plant, fine root is the central part consisting of the root system to contribute to plant water and nutrient taken up. However, the research on the molecular mechanism underlying fine root response to soil drought has not been well understood in cotton. To better characterize the proteomic changes of cotton fine roots under drought stress, 70±5% and 40±5% soil relative water content were designed as control (CK) and drought stress (DS) groups, respectively. Tandem mass tags (TMT) technology was used to determine the proteome profiles in fine roots. The proteomic differences between CK and DS were pairwise compared at 0, 30, and 45 days after drought stress (DAD). A total of 11,628 proteins were identified, of which 10,344 proteins contained quantitative information. According to the morphological, physiological, and biochemical characteristics, 30 and 45 DAD were selected as critical stages for further analysis. Results showed that 118 differentially expressed proteins (DEPs) were up-regulated and 105 down-regulated in DS 30 versus CK 30; 662 DEPs were up-regulated, and 611 were down-regulated in DS 45 versus CK 45. The DEP functions were determined for their classified pathways, mainly associated with carbohydrate metabolism, energy metabolism, fatty acid metabolism, amino acid metabolism, and secondary metabolite biosynthesis. DEPs related to phytohormone and stress/defense response were also identified. To verify the accuracy of the TMT results, 20 DEPs were randomly selected for parallel reaction monitoring (PRM) verification. And results showed that the quantitative results of TMT are consistent with those of PRM, which proved that the TMT results of this study are reliable. In this article we describe changes in the protein profiles occurring in response to drought stress in cotton fine roots. Proteomic analyses of plant responses to stressors could lead to the introduction of cotton cultivars with high resistance to drought stress. Such plants would be valuable for high yielding under drought as well as other unfavorable environmental conditions.

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Drought induced transcriptomic changes in tobacco

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