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Transcriptional profiling of the T. thermophilus HB8 response to tetracycline

ABSTRACT: We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 10 min after the addition of 50 mM tetracycline.

ORGANISM(S): Thermus thermophilus HB8

PROVIDER: GSE21432 | GEO | 2010/10/15

SECONDARY ACCESSION(S): PRJNA129483

REPOSITORIES: GEO

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Transcriptional profiling of the T. thermophilus HB8 response to tetracycline (low concentration)

Project description:We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 5 min after the addition of 10 mM tetracycline.

2010-10-15 | GSE21434 | GEO

Transcriptional profiling of the T. thermophilus HB8 response to tetracycline

Project description:We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 10 min after the addition of 50 mM tetracycline. Three wild-type strain of T. thermophilus HB8 were pre-cultured at 70 degC for 16 hours in 4 mL of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 mL) were inoculated into 1 L of the same medium and then cultivated at 70Â°C for 6 hours (A600 value being ~0.62). Then the 50 mM of tetracycline was added into the medium, and continued cultivation. Cells were collected at 0 and 10 min time point after the addition of tetracycline, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip.

2010-10-15 | E-GEOD-21432 | biostudies-arrayexpress

Transcriptional profiling of the T. thermophilus HB8 response to tetracycline (low concentration)

Project description:We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 5 min after the addition of 10 mM tetracycline. Three wild-type strain of T. thermophilus HB8 were pre-cultured at 70 degC for 16 hours in 3 mL of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 mL) were inoculated into 1 L of the same medium and then cultivated at 70Â°C for 5 hours (A600 value being ~0.47). Then the 10 mM of tetracycline was added into the medium, and continued cultivation. Cells were collected at 5 min time point after the addition of tetracycline, and then crude RNA was extracted. The expression of each mRNA was analyzed on a GeneChip.

2010-10-15 | E-GEOD-21434 | biostudies-arrayexpress

Transcriptional profiling of the T. thermophilus HB8 response to diamide

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Project description:We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 0 and 30 min after the addition of CuSO4.

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Differential expression analysis of T. thermophilus HB8 wild-type strain in response to iron

Project description:We observed the expression profile of the total mRNA in Thermus thermophilus HB8 wild-type strain at 0 and 30 min after the addition of FeSO4.

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