Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next Generation Sequencing of macrophages cocultured with tumor cell

ABSTRACT: To systematically examine the effect of tumor cells on macrophages, the RAW264.7 cells after a 24-hour coculture with CT26 cells were subjected to RNA sequencing, the complete medium of CT26 cells were used as control.

ORGANISM(S): Mus musculus

PROVIDER: GSE214450 | GEO | 2022/10/02

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Next Generation Sequencing analysis using SPOP mutant (F133V)-transduced RM-1-BM mouse prostate cancer cells and RAW264.7 mouse macrophage cells

Project description:In this experiment, we analyzed the gene expression profiles of SPOP mutant (F133V)-transduced (and vector control) RM-1-BM mouse prostate cancer cells and RAW264.7 macrophage cells in transwell coculture and monoculture in the context of olaparib (or vehicle) treatment by RNA sequencing.

2023-08-07 | GSE240199 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Fbl+/- Raw264.7 Transcriptomes

Project description:The goal of this study is to compare NGS-derived wild-type Raw264.7 transcriptome profiling (RNA-seq) to NGS-derived Fibrillarin knockdown (Fbl+/-) Raw264.7 RNA-seq. The mRNA profiles of wild-type Raw264.7 and Fbl+/− Raw264.7 were generated by deep sequencing, in duplicate, using illumina NovaSeq 6000.

2022-04-05 | GSE185660 | GEO

Multi-Enzymatic Limited Digestion - the Next-Generation Sequencing for Proteomics

Project description:An innovative approach merging both the bottom-up and middle-down ideologies to provide fast, robust and comprehensive solutions for proteomics studies. The methodology, referred to as MELD for Multiple-Enzymatic Limited Digestion, capitalizes on a single two-hour long proteolytic step involving the synergic action of a diluted mix of several enzymes. While the multi-enzymatic benefits are retained, the lower enzyme concentrations induce a limitation in the proteolytic reaction. This feature engenders numerous missed cleavage events and results in abundant peptides of diverse length with overlapping stretches of residues. This outcome eventually increases the extent and confidence in the sequence coverage while opening roads for refined and comprehensive protein characterization. We here use Adalimumab, a therapeutic ~144 kDa human immunoglobulin G IgG1 as a candidate molecule to compare the respective efficacy of state-of-the-art complete mono-enzymatic strategies encompassed in individual or combinational workflows with the standard MELD protocol for extensive protein characterization. The benchmarking analysis was performed over 12 Adalimumab samples issued from independent proteolytic digestions: 6 samples were processed using the MELD methodology, 3 with the combinatory methodology and 3 with the mono-enzymatic tryptic methodology. They were all subjected to independent database searches, using both constrained and unconstrained cleavage site rules for the latter.

2019-05-08 | PXD009800 | Pride

Gene expression profile at a single cell level of differentiating J1 mouse embryonic stem cells cocultured with OP9 cells

Project description:The OP9 coculture system is widely used to differentiate pluripotent stem cells into mesodermal lineages. We used single cell RNA sequencing to analyze differentiation trajectories and heterogeniety of differentiating pluripotent stem cells using the OP9 coculture system.

2024-07-10 | GSE271441 | GEO

Caco-2 cocultured with THP-1, time course

Project description:Previously, we constructed a coculture model to analyze the effect of macrophages on intestinal epithelial cells, and found that TNF-a secreted from human macrophage-like THP-1 cells induced cell damage to intestinal epithelial Caco-2 cells (Exp.Cell.Res. 2006, 312(19):3909-19). In this study, we present activation of NF-kB in Caco-2 cells within 15 min after coculturing. To reveal how TNF-a secreted from THP-1 cells affects Caco-2 cells in an early stage of coculture, we exhaustively analyzed the changes of gene expression in Caco-2 cells cocultured with THP-1 cells over the time periods of 0, 1, 3, 6, 24, and 48 h by using a DNA microarray. Differentially expressed genes extracted with maSigPro demonstrated that IEX-1 was the lowest p-value gene, that is, the most significantly changed gene among the up-regulated genes. The genes expressed in a similar pattern to IEX-1 involved immunity, apoptosis, and protein kinase cascade. These findings suggest that the stimuli of TNF-a from THP-1 cells activates NF-kB, leading induction of various gene expression. This pattern of gene expression indicates that not only early defense response but also cell death occurs at the same time, causing inflammatory condition.

2010-03-08 | GSE17625 | GEO

Caco-2 cocultured with THP-1, time course

2010-04-06 | E-GEOD-17625 | biostudies-arrayexpress

The proteome of P.denitrificans and S. oneidensis MR-1 when they cocultured

Project description:When S. oneidensis MR-1 was coculture with P. denitrificans, SMX degradation was largely enhanced. Therefore, the proteomic of S. oneidensis MR-1 and P. denitrificans when they were coculture to analysis the protein expression level of the two microorganisms compared with their pure culture in order to investigate the SMX degrading enhancing mechanism in protein level.

2021-08-12 | PXD027884 |

Next Generation Sequencing Facilitates Quantitative Analysis of sh-NC and sh-Etv5 macrophage line Raw264.7 Transcriptomes

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare sh-NC with sh-Etv5 macrophage line Raw264.7 transcriptome profiling (RNA-seq).

2022-02-06 | GSE166294 | GEO

Next Generation Protein Sequencing

Project description:NGPS is a method for de-novo, full-length protein sequencing in high throughput. The method is based on cleavage of the protein at semi-random sites by microwave-assisted acid hydrolysis (MAAH), enrichment of LC-MS/MS amenable peptides from the hydrolysate by solid-phase-extraction, LC-MS/MS analysis, de-novo long peptide tag sequencing of resulting peptides and assembly of peptide tags into consensus contigs.

2017-04-03 | PXD003804 | Pride

RNA-Seq analysis of LYN deficient HS-5 stroma cells cocultured with primary human CLL cells

Project description:LYN kinase is a tyrosine kinase, that regulates cellular homeostasis in a context-specific manner. Our group could show, that its expression in the leukemic microenvironment of chronic lymphocytic leukemia contributes to disease progression (Nguyen PH et al.; Cancer Cell; 2016). To analyze the effect of LYN kinase on the leukemia supportive phenotype of the bone marrow stromal cell line HS-5, we generated single cell clones of LYN deficient stroma cells. These cells were analyzed in a Multi-Omic approach, including ARNA-Seq of stromal cells after 72h of coculture with primary human chronic lymphocytic leukaemia (CLL) samples.

2023-02-10 | E-MTAB-12629 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data