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Expression data from mouse ES and iPS cells

ABSTRACT: Induced pluripotent stem (iPS) cells were produced from reprogramming of somatic cells, and they are shown to possess pluripotent properties similar to embryonic stem (ES) cells. Here we used microarrays to detail the global expression pattern among the ES cells and iPS cells, as well as the original mouse embryo fibroblast (MEF), to identify important players involved in the reprogramming process.

ORGANISM(S): Mus musculus

PROVIDER: GSE21515 | GEO | 2010/05/13

SECONDARY ACCESSION(S): PRJNA125973

REPOSITORIES: GEO

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Expression data from mouse ES and iPS cells

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Transcription profiling of mouse ES and iPS cells

Project description:Induced pluripotent stem (iPS) cells were produced from reprogramming of somatic cells, and they are shown to possess pluripotent properties similar to embryonic stem (ES) cells. Here we used microarrays to detail the global expression pattern among the ES cells and iPS cells, as well as the original mouse embryo fibroblast (MEF), to identify important players involved in the reprogramming process. Experiment Overall Design: Mouse ES cell cultures, as well as selected iPS cell lines and the original MEF cells they were derived from, were used for RNA extraction and hybridization on Affymetrix microarrays. Three biological replicates for each sample were processed. GeneChips were processed and data were analyzed as previously described (Zeng et al., Dev Biol 20004).

2009-08-08 | E-GEOD-16925 | biostudies-arrayexpress

Expression data from mouse ES and iPS cells

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2010-05-28 | E-GEOD-21515 | biostudies-arrayexpress

Genome-wide mapping of miRNAs expressed in embryonic stem cells and pluripotent stem cells generated by different reprogramming strategies

Project description:We report the miRNA profiling in MEF cells, ES cells and three Pluripotent Stem Cells obtained by three different reprogramming approaches from MEF cells based on Solexa sequencing. iPS cells are reprogrammed by four factors (OSKM) from MEF cells. NT-ESCs were established by reprogramming MEF cells into ESCs using nuclear transfer. NT-iPSCs were established to reflect the combination of nuclear transfer and iPS technologies. iPSCs, NT-ESCs, and NT-iPSCs were exactly derived from the same MEF cells. The results indicate NT-ESCs give expression to the unique miRNAs other than both ESCs and iPSCs while pluripotent cells acquire or retain the pluripotent specific miRNAs compared with MEF. Furthermore, the comparison of different reprogramming cells suggests that several miRNAs have key roles in distinctly developmental potential reprogrammine cells. Small RNA profiles of MEF, ES, iPS, NT-ES and NT-iPS cells were generated by Solexa sequencing. MEF and ES cells were performed in triplicate. iPS, NT-ES and NT-iPS cells were sequenced in duplicate.

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Transcriptome data for human ES, IMR90 and IMR90-derived iPS (ESIMR90) cells

2011-01-05 | GSE26453 | GEO

Transcriptome data for human ES, FORESKIN and FORESKIN-derived iPS (ES4SKIN) cells

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Transcriptome data for human ES, FORESKIN and FORESKIN-derived iPS (ES4SKIN) cells

Project description:Assessing relevant differences between human induced pluripotent stem (iPS) cells and human embryonic stem (ES) cells is important, given that such differences may impact their potential therapeutic use. We used microarray to profile the changes in global gene expression arising from the reprogramming of somatic cells to induced pluripotent stem cells. Comparison of gene expression between human embryonic stem cells (HES-3), induced pluripotent stem cells (ES4SKIN) and its parental fibroblasts (FORESKIN). A pair of biololical replicates were analysed for each cell line.

2011-01-05 | E-GEOD-26451 | biostudies-arrayexpress

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2011-01-05 | E-GEOD-26453 | biostudies-arrayexpress

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2010-08-18 | E-GEOD-17004 | biostudies-arrayexpress

Expression data from iPS and human ES cells

Project description:Assays to assess the quality of the reprogramming of human Induced pluripotent stem cells are needed. We have previously shown that hESC can be differentiated into embryonic and fetal type of red blood cells that sequentially express three types of hemoglobins recapitulating early human erythropoiesis. We report here that we have produced iPS from three somatic cell types: adult skin fibroblasts as well as embryonic and fetal mesenchymal stem cells. We show that some of these iPS are fully reprogrammed into a pluripotent state that is undistinguishable from that of hESCs based and low and high-throughput expression analysis and detailed expression of globin expression patterns suggesting that reprogramming with the four original Yamanaka pluripotency factors leads to complete erasure of all functionally important epigenetic marks associated with hematopoietic differentiation regardless of the age or the tissue type of the donor cells. We also report that reprogramming can also lead to abnormal iPS that are undistinguishable from hES cells by morphology, and by the expression of their endogenous genes but that are grossly abnormal in their differentiation potential. The most likely cause of abnormal reprogramming is failure to silence the virally transduced reprogramming factors. The ability to produce large number of erythroid cells with embryonic and fetal-like characteristics is likely to have many translational applications the gene expression in different source of iPS and standard ES cells H1

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