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Longitudinal Analysis of Influenza Vaccines on DNA methylation Identifies the RIG-I Signaling Pathway

ABSTRACT: Influenza virus infection was previously found to alter the promoter DNA methylation of key immune response-related genes, including type-1 interferons and proinflammatory cytokines. However, less is known about the effect of the influenza vaccine on the epigenome. Therefore, we utilized a targeted DNA methylation to study the longitudinal effects (day 0 pre-vaccination and day 28 post-vaccination) of influenza vaccination responses in peripheral blood mononuclear cells. We found that baseline methylation profiles are associated with protective immunity and immune conversion prior to influenza vaccination. Moreover, we identified 481 differentially methylated sites 28 days post-vaccination. These sites were enriched for genes involved in the regulation of the RIG-I signaling pathway, an important regulator of viral response. Our results suggest that DNA methylation changes to components of the RIG-1 pathway may impact vaccine effectiveness.

ORGANISM(S): Homo sapiens

PROVIDER: GSE215157 | GEO | 2023/07/31

REPOSITORIES: GEO

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