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Human iPSC-derived renal collecting duct organoids model cystogenesis in ADPKD

ABSTRACT: Here, establishing expansion cultures of hiPSC-derived ureteric bud tip cells, an embryonic precursor that gives rise to collecting ducts, we succeeded in advancing the developmental stage of collecting duct organoids and showed that all collecting duct organoids derived from PKD1-/- hiPSCs spontaneously develop multiple cysts, clarifying the initiation mechanisms of cystogenesis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE215875 | GEO | 2023/11/30

REPOSITORIES: GEO

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Human iPSC-derived renal collecting duct organoids model cystogenesis in ADPKD

Project description:We established the expansion culture of human iPSC-derived ureteric bud tip cells (UBTCs), an embryonic precursor that give rise to collecting ducts (CDs), and succeeded in advancing the developmental stage of CD organoids. We used single cell RNA-sequencing (scRNA-seq) to dissect cell types in CD organoids.

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