Transcriptomics

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Expansion of human iPSC derived ureteric bud organoids with repeated branching potential


ABSTRACT: We report a new method to generate UB organoids that possess epithelial polarity and tubular lumen and repeat branching morphogenesis. We also succeeded in monitoring UB tip cells by utilizing the ability of tip cells to uptake very-low-density lipoprotein, cryopreserving UB progenitor cells and expanding UB tip cells that can reconstitute the organoids and differentiate into collecting duct progenitors. Moreover, we successfully reproduced some phenotypes of multicystic dysplastic kidney (MCDK) using the UB organoids.

ORGANISM(S): Homo sapiens

PROVIDER: GSE152685 | GEO | 2020/07/15

REPOSITORIES: GEO

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