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Transcriptome sequencing in patients with Tetralogy of Fallot and healthy unaffected individuals

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE217773 | GEO | 2023/03/13

REPOSITORIES: GEO

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Transcriptome sequencing in patients with Tetralogy of Fallot and healthy unaffected individuals [miRNA]

Project description:The right ventricular tissues of 5 children with tetralogy of Fallot and 5 healthy unaffected individuals were collected and sequenced in full transcriptome. Mechanism of tetralogy of fallot and cerna regulatory network were analyzed.

2023-03-13 | GSE217771 | GEO

Transcriptome sequencing in patients with Tetralogy of Fallot and healthy unaffected individuals [LncRNA/mRNA]

2023-03-13 | GSE217772 | GEO

Small RNA sequencing in patients with Tetralogy of Fallot and healthy unaffected individuals

Project description:Right ventricular small RNA profiles from 22 patients with Tetralogy of Fallot (TOF) and small RNA-seq profiles from the left and right ventricle (LV and RV, respectively) of 4 healthy unaffected individuals (NH) were generated. The total RNA was isolated from the 30 human heart samples using TRIzol. Small RNAs were isolated from total RNA and prepared for microRNA sequencing using Illumina Kit FG-102-1009 according to the manufacturer's protocol (Preparing Samples for Analysis of Small RNA Illumina 2007). The sequencing libraries were generated using a non-strand-specific library construction method. The purified DNA fragments were used directly for cluster generation, and 36 cycles of single-end read sequencing were performed using the Illumina Genome Analyzer. Sequencing reads were extracted from the image files using the open source Firecrest and Bustard applications (Illumina Genome Analyzer pipeline 1.3.2).

2019-12-04 | GSE36759 | GEO

mRNA expression profiling in patients with Tetralogy of Fallot and healthy unaffected individuals

2014-02-04 | GSE36761 | GEO

mRNA expression profiling in patients with Tetralogy of Fallot and healthy unaffected individuals

Project description:Right ventricular mRNA profiles from 22 patients with Tetralogy of Fallot (TOF) and mRNA-seq profiles from the left and right ventricle (LV and RV, respectively) of 4 healthy unaffected individuals (NH) were generated. The total RNA was isolated from the 30 human heart samples using TRIzol. mRNAs were isolated from total RNA and prepared for sequencing using Illumina Kit RS-100-0801 according to the manufacturer's protocol (Preparing Samples for Sequencing of mRNA Sept 2008). The sequencing libraries were generated using a non-strand-specific library construction method. The purified DNA fragments were used directly for cluster generation, and 36 cycles of single-end read sequencing were performed using the Illumina Genome Analyzer. Sequencing reads were extracted from the image files using the open source Firecrest and Bustard applications (Illumina Genome Analyzer pipeline 1.3.2, 1.4.0 and 1.5.0). Right ventricular mRNA-seq profiles from 22 patients with Tetralogy of Fallot and mRNA-seq profiles from the left and right ventricle of 4 healthy unaffected individuals. Supplementary processed data files: GSE36761_gene_expression_levels.txt: Gene expression profiling data for all samples. GSE36761_gene_expression_levels_normalized.txt: Normalized gene expression profiling data for all samples. GSE36761_transcript_expression_levels.txt: Transcript expression profiling data for all samples. GSE36761_transcript_expression_levels_normalized.txt: Normalized transcript expression profiling data for all samples. Genome build: hg18

2014-02-04 | E-GEOD-36761 | biostudies-arrayexpress

DNA sequencing of TGFβ2 in sporadic patients with tetralogy of Fallot.

Project description:Transforming growth factor β2 (TGFβ2) plays an essential role in cardiac morphogenesis. However, the prevalence of TGFβ2 mutations in congenital heart disease (CHAD) and the correlation between the TGFβ2 genotype and the CHAD phenotype have not been studied extensively. The aim of this study was to examine DNA sequence changes in the TGFβ2 gene in sporadic patients with tetralogy of Fallot (TOF), and to observe whether TGFβ2 is the susceptibility gene for TOF. A cohort of 100 pediatric patients with TOF was recruited to the study; 200 healthy children were used as controls. PCR and genotyping were conducted for the detection of DNA changes in TGFβ2. The exons and the 5' untranslated region (5'UTR) sequences of the TGFβ2 gene were amplified. No mutations were identified in the coding region in any of the TOF patients. However, three single nucleotide changes, including 9126 A>AC, 9353 A>AG and 9040_9043 del CTTC, in the 5'UTR were found. There were no significant differences in allelic frequencies and genotype frequencies of position 9126 and 9353 between the TOF group and the control group. On the contrary, a significant difference was identified in the allelic frequencies (χ(2)=17.469, P<0.001) of position 9040_9043 in the 5'UTR between the TOF group and the control group. Our results suggest that TGFβ2 may be a potential candidate gene of TOF. SNPs at position 9040_9043 del CTTC in the 5'UTR of TGFβ2 may be associated with susceptibility to TOF. The CTTC allele may be the susceptibility allele for TOF. However, the exact effect of these sequence changes requires further study using functional experiments.

| S-EPMC3438627 | biostudies-literature

Expression data in Heart Tissue associated with Tetralogy of Fallot

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-03-01 | GSE146220 | GEO

Noncoding RNA expression in myocardium from infants with tetralogy of Fallot

Project description:This SuperSeries is composed of the SubSeries listed below.

2012-07-12 | GSE35781 | GEO

Controlling Reoxygenation During Cardiopulmonary Bypass is Associated with Reduced Transcriptomic Changes in Cyanotic Patients with Tetralogy of Fallot Undergoing Heart Surgery

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2012-12-06 | E-GEOD-38177 | biostudies-arrayexpress

Controlling Reoxygenation During Cardiopulmonary Bypass is Associated with Reduced Transcriptomic Changes in Cyanotic Patients with Tetralogy of Fallot Undergoing Heart Surgery

Project description:This SuperSeries is composed of the SubSeries listed below.