Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mapping of genome-wide histone modifications in SGF29 knockout vs NTC U937 AML cells. Mapping of binding of SGF29 and Tudor-domain mutant SGF29_D196R by FLAG ChIP [ChIP-seq SGF29]

ABSTRACT: ChIP-seq was performed to investigate the genomic binding of SGF29 in U937 cells using FLAG-tagged SGF29-wild-type or SGF29_D196R mutant overexpression constructs. To assess the effects SGF29 in histone mark deposition, ChIP-seq was performed for different histone modifications in U937 cells with CRISPR-knockout of SGF29 or the expression of a control sgRNA. We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 different cells at two time points.

ORGANISM(S): Homo sapiens

PROVIDER: GSE217777 | GEO | 2023/11/10

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Mapping of genome-wide histone modifications in SGF29 knockout vs NTC U937 AML cells. Mapping of binding of SGF29 and Tudor-domain mutant SGF29_D196R by FLAG ChIP [ChIP-seq SGF29]

Project description:Mapping of genome-wide histone modifications in SGF29 knockout vs NTC U937 AML cells. Mapping of binding of SGF29 and Tudor-domain mutant SGF29_D196R by FLAG ChIP [ChIP-seq SGF29]

| PRJNA900361 | ENA

High Density Domain-Focused CRISPR Screens Reveal Novel Epigenetic Regulators of HOX/MEIS Activation in Acute Myeloid Leukemia [RNA-seq, SGF29 KO vs. NTC in MOLM13 and U937]

Project description:To investigate the transcriptomic effects upon loss of SGF29 in the AML cell lines U937 and MOLM13, we performed CRISPR knockouts, as well as transduction with non-targeting controls, and harvested samples for bulk RNA-Seq.

2024-10-31 | GSE243935 | GEO

Effect of CRISPR knockout of MLL, CSNK2A1 and SGF29 on gene expression profiles in acute myeloid leukemia U937 cells [RNA-seq]

Project description:To investigate the transcriptomic effects upon loss of CSNK2A1 (Casein kinase 2, CK2), MLL and SGF29 in the AML cell line U937 we performed CRISPR knockouts, as well as transduction with non-targeting controls, and harvested samples for bulk RNA-Seq. We then performed gene expression profiling analysis using data obtained from RNA-seq of the different CRISPRko perturbations compared to non-targeting controls.

2023-11-10 | GSE217776 | GEO

U937 ctrl vs U937 Saha (Vorinostat; suberoylanilide hydroxamic acid) 6h

Project description:miRNA expression profiling of U937 ctrl vs U937 treated Saha 5 µM 6h

2017-05-22 | GSE69957 | GEO

U937 scramble (Usc) vs U937 miR-194-5p (UmiR-194-5p)

Project description:Transcriptional profiling of U937 scramble vs U937 miR-194-5p (UmiR-194-5p)

2017-05-22 | GSE73646 | GEO

Znf143 binding sites in U937 cells by ChIPexo

Project description:We performed ChIPexo sequencing to identify the binding sites of Znf143 in the U937 cell line.

2017-01-01 | GSE65130 | GEO

Epigenome analysis of U937, U937 treated with 5mM azacytidine for 24 and 72 hours and its azacytidine-resistant derivative

Project description:Genome-wide DNA methylation profiling of U937 and its azacytidine-resistant derivative R-U937.

2013-07-01 | GSE45707 | GEO

Gene expression analysis of U937 cells during the differentiation by phorbol 12-myristate 13-acetate (PMA)

Project description:U937 cells, human lyphoma cell line, differentiate to the macrophage-like cells by the phorbol 12-myristate 13-acetate (PMA). We investigated the regulation mechanisms of gene expression during the U937 differentiation by RNA-seq.

2020-11-30 | GSE155459 | GEO

Transcription profiling of human U937 cells expressing AML1/ETO compared to vector transfected U937 cells to identify genes regulated by AML1/ETO

Project description:Approximately 20% of Acute Myelogenous Leukemia (AML) cases carry the t(8;21) translocation, which involves the AML1 and ETO genes, and express the resulting AML1/ETO fusion protein that functions as a transcriptional repressor by recruiting NCoR/SMRT/HDAC complexes to DNA. We used microarrays to identify genes differentially expressed in U937 cells expressing AML1/ETO compared to vector transfected U937 cells. Experiment Overall Design: A U937 cell line that conditionally expresses HA-tagged AML1/ETO under the control of the mouse metallothionine promoter (U937-A1E) (Alcalay et al., J.Clin.Invest, 2003,112, 1751-1761) was used. A cell line caryying the empty vector was used as control Cells were treated for 8h with 100uM ZnSO4 to induce transgene expression. For each of the U937 cell lines (AML1/ETO and Mt), three independent RNA extractions were performed, and an equal quantity of each of the three RNA preparations was then mixed to generate an RNA pool for each sample.

2009-01-13 | E-GEOD-10520 | biostudies-arrayexpress

U937 miR-194-5p (UmiR-194-5p) vs U937 miR-194-5p (UmiR-194-5p) treated with MS275 (SNDX 275;Entinostat)

Project description:Transcriptional profiling of U937 miR-194-5p (UmiR-194-5p) vs U937 miR-194-5p (UmiR-194-5p) treated with MS275 (SNDX 275;Entinostat) for 24 h at 5uM concetration

2017-05-22 | GSE73647 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data