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Transcriptome sequencing of A549 cells

ABSTRACT: We analyzed the global effect of SOX9 knockdown and TGFB1 treatment by sequencing the transcriptomes of A549 cells transfected with siSOX9 or control siRNA and treated with TGFB1.

ORGANISM(S): Homo sapiens

PROVIDER: GSE217880 | GEO | 2024/01/15

REPOSITORIES: GEO

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Chromatin profiling of A549 cells

Project description:We analyzed SOX9 chromatin binding upon TGFB1 treatment as well as the global effect of SOX9 knockdown and TGFB1 treatment by ChIP-sequencing and ATAC-sequencing of A549 cells transfected with siSOX9 or control siRNA and treated with TGFB1.

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Transcriptome sequencing of K-562 cells

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Expression data from KEAP1 overexpression and NRF2 siRNA knockdown of A549 NSCLC cell

Project description:KEAP1 overexpressed and NRF2 siRNA knockdown A549 NSCLC cells were used to identify downstream genes of NRF2 pathway separately and by combinatorial analysis. We used triplicate microarrays of transfected A549 cells with mKeap1-GFP for overexpression, siRNAs targeting NRF2 for knockdown and siGFP as control respectively. As a result, we identified several genes which are involved in cancer metabolic functions in these cells. We used microarrays to identify the gene downregulated in both KEAP1 overexpressed and NRF2 siRNA knockdown A549 NSCLC cells and found a subset of downregulated genes which are involved in metabolic functions.

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Genome-wide transcriptional profiling of Legionella Pneumophila-infected A549 cells transfected with siRNA against TNFAIP2

Project description:In this study we transfected A549 cells with siRNA against TNFAIP2, infected them with L. pneumophila and performed transcriptional profiling. We found enrichment of genes in pro-inflammatory pathways by Pathway Over-respresentation analysis upon infection. There was no significant change in gene expression that we could attribute specifically to the knockdown of TNFAIP2. Examination of the transcriptional response of A549 cells to Legionella infection with concomitant TNFAIP2 knockdown

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Identification of Nrf2-regulated genes in A549 lung cancer cells by oligonucleotide microarray

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Knockdown TIMM13 in A549 lung cancer cell line

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