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Genome-wide transcriptional profiling of Legionella Pneumophila-infected A549 cells transfected with siRNA against TNFAIP2

ABSTRACT: In this study we transfected A549 cells with siRNA against TNFAIP2, infected them with L. pneumophila and performed transcriptional profiling. We found enrichment of genes in pro-inflammatory pathways by Pathway Over-respresentation analysis upon infection. There was no significant change in gene expression that we could attribute specifically to the knockdown of TNFAIP2. Examination of the transcriptional response of A549 cells to Legionella infection with concomitant TNFAIP2 knockdown

ORGANISM(S): Homo sapiens

SUBMITTER: Brian Caffrey

PROVIDER: E-GEOD-80214 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide transcriptional profiling of Legionella Pneumophila-infected A549 cells transfected with siRNA against TNFAIP2

Project description:In this study we transfected A549 cells with siRNA against TNFAIP2, infected them with L. pneumophila and performed transcriptional profiling. We found enrichment of genes in pro-inflammatory pathways by Pathway Over-respresentation analysis upon infection. There was no significant change in gene expression that we could attribute specifically to the knockdown of TNFAIP2.

2016-04-13 | GSE80214 | GEO

Strand specific sequencing illustrates the complex transcriptional response of Legionella pneumophila during infection

Project description:Legionella pneumophila is a Gram-negative facultative intracellular human pathogen with a distinct biphasic lifestyle. One of its primary environmental hosts in the free-living amoeba Acanthamoeba castellanii and its infection by L. pneumophila mimics that seen in human macrophages. Here we present analysis of strand specific sequencing of the transcriptional response of L. pneumophila in broth growth and in infection of A. castellanii. Examination of 2 infection and 3 broth growth time points

2011-02-11 | E-GEOD-27232 | biostudies-arrayexpress

Transcription profiling by array of Legionella pneumophila ppGpp knockout and relA over expression mutants.

Project description:Effect of ppGpp on transcript level after RelA overexpression (90 min). The cells are constructed in our lab. They are knock-out mutants in the production of ppGpp(guanosine tetraphosphate) of Legionella, complemented with an over-expression vector containing the relA gene to express the RelA protein (responsible for the ppGpp production). The aim is to follow the gene response in Legionella pneumophila after induction of ppGpp at different time points.

2010-04-14 | E-MTAB-211 | biostudies-arrayexpress

Legionella pneumophila transcriptomic response to CuO nanoparticles

Project description:Legionella pneumophila was exposed for 3h to 160 ug/ml of CuO nanoparticle. Untreated samples are used as the control condition.

2013-03-04 | GSE41966 | GEO

RNAseq profiling of a lpp1663 mutant compared to its isogenic parent Legionella pneumophila strain Paris

Project description:Comparaison of the transcriptional profile of mutant of the lpp1663 gene and the parent strain Legionella pneumophila strain Paris. lpp1663 codes for a protein with a ProQ domain (PFAM PF04352). A mutant of this gene was obtained by deleting the lpp1663 ORF and replacing it with a gene confering resistance to kanamycin. Transcriptional profiling was done on cultures grown to exponential phase (OD=0.8). Three independent biological replicates were analysed.

2020-09-01 | E-MTAB-4095 | biostudies-arrayexpress

RNAseq profiling of a lpp0148 mutant compared to its isogenic parent Legionella pneumophila strain Paris

Project description:Comparaison of the transcriptional profile of mutant of the lpp0148 gene and the parent strain Legionella pneumophila strain Paris. lpp0148 codes for a protein with a ProQ domain (PFAM PF04352). A mutant of this gene was obtained by inserting a premature stop codon in the lpp0148 ORF. The lpp0148 mutant is constitutively competent for natural transformation as described here http://www.ncbi.nlm.nih.gov/pubmed/26526572. Transcriptional profiling was done on cultures grown to exponential phase (OD=0.8). Three independent biological replicates were analysed.

2016-05-24 | E-MTAB-4094 | biostudies-arrayexpress

Transcription profiling of Dictyostelium discoideum wild type and dupA null after infection with Legionella pneumophila

Project description:The social amoeba Dictyostelium discoideum is vulnerable to infection by the pathogen that causes Legionnaire's Disease, Legionella pneumophila. Dictystelium cells lacking the dual-specificity phospahatase DupA are at least partially resistant to infection, and strikingly the expression profile of uninfected dupA null cells mirrors the profile of infected wild-type cells to a considerable extent. This suggests that DupA has a key role in regulating host defence systems.

2009-09-24 | E-TABM-509 | biostudies-arrayexpress

Legionella pneumophila

Project description:Legionella pneumophila

| PRJNA526503 | ENA

Legionella pneumophila transcriptomic response of hfq mutant during post-exponential phase

Project description:Legionella pneumophila wild-type and hfq mutant were grown to post-exponential. The wild-type strain is used as the control.

2013-05-21 | GSE42905 | GEO

Legionella pneumophila proteome

Project description:Legionella pneumophila cells were harvested during exponential growth (RP) and stationary growth (TP). VBNC cells were also anylzed. Protein subfractions were studied.

2015-11-13 | PXD001926 | Pride

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