Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Epigenome-wide Association Study of Obesity Susceptibility in Human Twins

ABSTRACT: The purpose of the current study was to use an epigenome-wide association approach (EWAS) to identify a potential DNA methylation alterations associated with obesity using twins discordant on obesity status. Buccal cells (from a cheek swab) were used as a purified marker cell for the epigenetic analysis. Analysis of differential DNA methylation regions (DMRs) was used to identify epigenetic associations with metabolic and dietary measures in obesity parameters discordant twins. An edgeR analysis provided a DMR signature with p<1e-04, but a false discovery rate analysis demonstrated a lack of significance due to low sample size and known multivariant origins of obesity.

ORGANISM(S): Homo sapiens

PROVIDER: GSE218363 | GEO | 2023/02/18

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Epigenome-wide Association Study of Physical Activity and Physiological Parameters in Discordant Monozygotic Twins

Project description:An epigenome-wide association study (EWAS) was performed on buccal cells from monozygotic-twins (MZ) reared together as children, but who live apart as adults. Cohorts of twin pairs were used to investigate associations between neighborhood walkability and objectively measured physical activity (PA) levels. Due to dramatic cellular epigenetic sex differences, male and female MZ twin pairs were analyzed separately to identify differential DNA methylation regions (DMRs). A priori comparisons were made on MZ twin pairs discordant on body mass index (BMI), PA levels, and neighborhood walkability. In addition to direct comparative analysis to identify specific DMRs, a weighted gene coexpression network analysis (WGCNA) was performed to identify DNA methylation sites associated with the physiological traits of interest. The pairs discordant in PA levels had epigenetic alterations that correlated with reduced metabolic parameters (i.e., BMI). The DNA methylation sites are associated with over fifty genes previously found to be specific to vigorous PA, metabolic risk factors, and sex. Combined observations demonstrate that behavioral factors, such as physical activity, appear to promote systemic epigenetic alterations that impact metabolic risk factors. The epigenetic DNA methylation sites and associated genes identified provide insight into PA impacts on metabolic parameters and the etiology of obesity.

2022-12-01 | GSE216387 | GEO

Microbial RNAseq analysis of cecal and fecal samples collected from mice colonized with the microbiota of human twins discordant for obesity

Project description:Microbial RNAseq analysis of cecal and fecal samples collected from mice colonized with the microbiota of human twins discordant for obesity. Samples were colleted at the time of sacrifice, or 15 days after colonization from mice gavaged with uncultured or cultured fecal microbiota from the lean twins or their obese co-twins. Samples were sequenced using Illumina HiSeq technology, with 101 paired end chemistry.

2013-10-01 | GSE48861 | GEO

Transcription profiling of fat biopsies from monozygotic twins discordant for body mass index to elucidate the effects of acquired obesity

Project description:Elucidating the effects of acquired obesity by comparison of fat biopsies from monozygotic twins discordant for BMI.

2008-02-15 | E-MEXP-1425 | biostudies-arrayexpress

NSCLP-Twins

Project description:this study analyzes the WGBS of twins discordant for non syndromic cleft lip and palate

2021-04-24 | GSE173211 | GEO

Gene expression analysis in MZ twins discordant for IAR

Project description:This SuperSeries is composed of the following subset Series: GSE37146: Gene expression analysis in MZ twins discordant for IAR [in vitro] GSE37155: Gene expression analysis in MZ twins discordant for IAR [in vivo] Refer to individual Series

2012-04-14 | E-GEOD-37156 | biostudies-arrayexpress

Microbial RNAseq analysis of cecal and fecal samples collected from mice colonized with the microbiota of human twins discordant for obesity

2013-10-01 | E-GEOD-48861 | biostudies-arrayexpress

Gene expression analysis in MZ twins discordant for IAR [in vitro]

Project description:Gene expression analysis in CD4+ T cells extracted from allergen-challenged PBMCs, isolated from discordant MZ twins with IAR MZ twins discordant for intermittent allergic rhinitis (IAR)

2012-04-14 | E-GEOD-37146 | biostudies-arrayexpress

Gene expression analysis in MZ twins discordant for IAR [in vivo]

Project description:Gene expression analysis in CD4+ T cells extracted from PBMCs, isolated from discordant MZ twins with IAR during season MZ twins discordant for intermittent allergic rhinitis (IAR)

2012-04-14 | E-GEOD-37155 | biostudies-arrayexpress

Epigenetic assimilation in the aging human brain

Project description:Epigenetic misregulation is consistent with the various non-Mendelian features of Alzheimer's disease (AD) and may even play an etiological role in the disease. We performed DNA modification profiling of post-mortem brain samples from twins who differed for age of AD onset and buccal samples from twins who were discordant for AD at the time of sample collection. We performed DNA methylome analysis on frontal cortex, cerebellum, or buccal tissues from monozygotic twins and dizygotic twins discordant for Alzheimerâs disease (AD) or disease age of onset. DNA samples were enriched for unmodified fraction of the genome using DNA-modification sensitive restriction enzyme digestion followed by adaptor-mediated PCR. The enriched fractions were labelled with a fluorescent dye (Cy3) and hybridized onto the array with a common reference pool (Cy5).

2016-04-04 | E-GEOD-61242 | biostudies-arrayexpress

Phenotypically concordant and discordant monozygotic twins display different DNA copy number variation profiles (BAC)

Project description:The exploration of copy number variation (CNV), notably of somatic cells, is an understudied aspect of genome biology. Any differences in the genetic make-up between twins derived from the same zygote represent an extreme example of somatic variation. We studied 19 pairs of monozygotic twins with either concordant or discordant phenotype using two platforms for genome-wide CNV analyses and show that CNVs exist within pairs in both groups. These findings impact our views of genotypic and phenotypic diversity in monozygotic twins, and suggest that CNV analysis in phenotypically discordant monozygotic twins may provide a powerful tool in identifying disease predisposition loci. Our results also imply that caution should be exercised with the interpretation of disease causality of de novo CNVs found in patients based on analysis of a single tissue in routine disease-related DNA diagnostics Keywords: copy number variation, concordant and discordant monozygotic twins

2007-12-11 | GSE9559 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data