Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Architecture and function of lamellar cells in Meissner corpuscle

ABSTRACT: Meissner corpuscles detect transient touch and vibration in glabrous skin, but their ultrastructure and mechanism of function are poorly understood. The Meissner corpuscle sensory core contains the terminal neurite(s) of the mechanoreceptor afferent surrounded by Schwann cell-derived lamellar cells. The afferent is thought to be the sole touch-sensing element within the corpuscle, whereas the role of lamellar cells is unclear. Here, we present a high-resolution three-dimensional ultrastructure of an avian Meissner (Grandry) corpuscle in intact skin acquired using the enhanced focused ion beam scanning electron microscopy (FIB-SEM), followed by machine learning based segmentation and reconstruction. We show that the afferent splits within the corpuscle into several disk-shaped endings layered between flattened lamellar cells. Each lamellar cell contains numerous exocytotic dense core vesicles and forms large-area contacts with adjacent afferent disks. Together with single-corpuscle RNA sequencing and electrophysiology, our FIB-SEM data reveal lamellar cells as secretory sensors of touch. We developed a method for direct electrophysiological recordings of afferent activity from an individual corpuscle in the skin and show that activation of a single mechanosensitive lamellar cell is sufficient to trigger action potentials in the afferent. These results reveal comprehensive Meissner (Grandry) corpuscle architecture and demonstrate a dual sensory mechanism comprised of a mechanoreceptor afferent and lamellar cells.

ORGANISM(S): Anas platyrhynchos

PROVIDER: GSE218686 | GEO | 2023/09/21

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Structural and functional dissection of Pacinian corpuscle reveals a multicellular mechanism of touch detection independent of the outer core

Project description:Lamellated corpuscles are rapidly adapting mechanoreceptors that detect transient touch and high-frequency vibration in many vertebrates. It the prevailing model, these properties are determined by outer core, which acts as a mechanical filter that limits static and low-frequency stimuli from reaching afferent terminal—the sole site of touch detection in corpuscles. Using focused ion beam scanning electron microscopy and single corpuscle electrophysiology, we determine the detailed architecture of corpuscular components and reveal their contribution to touch detection. We show that outer core is dispensable for rapid adaptation and frequency tuning. Instead, these properties are encoded within inner core, composed of gap junction-coupled syncytium formed by lamellar Schwann cells (LCS) around afferent terminal. By acting as additional touch sensors, LSCs potentiate mechanosensitivity of the terminal, which detects touch via fast-inactivating ion channels. Our findings support a model in which lamellated corpuscle function is mediated by a bicellular mechanism within inner core.

2024-10-08 | GSE273272 | GEO

Skin-type-dependent development of murine mechanosensory neurons

Project description:Mechanosensory neurons innervating the skin underlie our sense of touch. Fast-conducting, rapidly adapting mechanoreceptors innervating glabrous (non-hairy) skin form Meissner corpuscles, while in hairy skin, they associate with hair follicles, forming longitudinal lanceolate endings. How mechanoreceptors develop axonal endings appropriate for their skin targets is unknown. We report that mechanoreceptor morphologies across different skin regions are indistinguishable during early development but diverge post-natally, in parallel with skin maturation. Neurons terminating along the glabrous and hairy skin border exhibit hybrid morphologies, forming both Meissner corpuscles and lanceolate endings. Additionally, molecular profiles of neonatal glabrous and hairy skin-innervating neurons largely overlap. In mouse mutants with ectopic glabrous skin, mechanosensory neurons form end-organs appropriate for the altered skin type. Finally, BMP5 and BMP7 are enriched in glabrous skin, and signaling through type I bone morphogenetic protein (BMP) receptors in neurons is critical for Meissner corpuscle morphology. Thus, mechanoreceptor morphogenesis is flexibly instructed by target tissues.

2023-07-27 | GSE229111 | GEO

Architecture and function of lamellar cells in Meissner corpuscle

Project description:Architecture and function of lamellar cells in Meissner corpuscle

| PRJNA904802 | ENA

Characterization of human Merkel cells and their response to Merkel cell polyomavirus

Project description:Merkel cells are epidermal mechanoreceptor cells responsible for the perception of gentle touch. Merkel cell carcinoma (MCC) is a rare and highly aggressive skin cancer. Although MCC histologically resembles Merkel cells, the cell of origin for MCC is unknown. MCC frequently contains integrated Merkel cell polyomavirus (MCPyV), a small DNA tumor virus with widespread prevalence. Whether MCPyV can transform Merkel cells is unknown. Here, we describe the isolation and long-term expansion of human Merkel cells from neonatal foreskin. We validated the expression of several Merkel cell-related factors by RNASeq, and assessed the ultrastructure by electron microscopy. Culture of Merkel cell preparations on an artificial basement membrane promoted the formation of structures containing both Merkel and non-Merkel cell populations. To determine whether Merkel cells were susceptible to transformation, we expressed tumor-derived MCPyV T antigens and additional oncogenes. We were unable to demonstrate tumorigenesis in immunodeficient mice, but were able to detect T antigen expression from excised cells weeks after implantation. These results highlight that foreskin-isolated Merkel cells can be propagated extensively, sustain expression of MCPyV T antigens, but are not susceptible to transformation by MCPyV, suggesting that Merkel cells from non-glabrous skin may not be a cell of origin for MCC.

2017-04-01 | GSE79657 | GEO

Lamellar cells from Pacinian and Meissner corpuscles are touch sensors

Project description:Lamellar cells from Pacinian and Meissner corpuscles are touch sensors

| PRJNA650145 | ENA

Merkel Cells Activate Sensory Neural Pathways through Adrenergic Synapses

Project description:Epithelial-neuronal signaling is essential for sensory encoding in touch, itch and nociception; however, little is known about the release mechanisms and neurotransmitter receptors through which skin cells govern neuronal excitability. Merkel cells are mechanosensory epidermal cells that have long been proposed to activate neuronal afferents through chemical synaptic transmission. We employed a set of classical criteria for chemical neurotransmission as framework to directly test this hypothesis. RNA sequencing of adult Merkel cells demonstrated that they express presynaptic molecules and biosynthetic machinery for adrenergic transmission. Moreover, live-cell imaging directly demonstrated that Merkel cells mediate activity- and VMAT-dependent release of fluorescent catecholamine neurotransmitter analogues. Touch-evoked firing in Merkel-cell afferents was inhibited either by pre-synaptic silencing of SNARE-mediated vesicle release from Merkel cells or by neuronal deletion of b2-adrenergic receptors. Together, these results identify both pre- and postsynaptic mechanisms through which Merkel cells excite mechanosensory afferents to encode gentle touch.

2018-11-08 | GSE114336 | GEO

Transcription profiling of mouse dorsal root ganglion

Project description:The molecular identity of touch sensation is still largely unknown. We choose an extrem and very specific example, touch sensation of pennis glan, to study this problem. It's known from previous retro-grade labeling result that only one DRG in rat innervate the pennis glan, which makes it idea for microarry analysis. To clone the mechanosensory receptor specifically or highly expressed in the primary sensory neurons innervating pennis glan. There is a specific mechanosensory receptor in the primary sensory neurons innervating pennis glan. 1. Retrograde labeling the innervating nerve by DiI; 2. Dissect DRGs, dissociate neurons and pick up the fluorescent cells. 3. Pool around 20 positive cells and 50 control cells (big diameter neuron and small to medium diameter neuron respectively), purify total RNA. 4. Two rounds of amplification; 5. Affymetrix analysis

2008-06-11 | E-GEOD-11207 | biostudies-arrayexpress

Dorsal root ganglion (katz-affy-mouse-210210)

Project description:The molecular identity of touch sensation is still largely unknown. We choose an extrem and very specific example, touch sensation of pennis glan, to study this problem. It's known from previous retro-grade labeling result that only one DRG in rat innervate the pennis glan, which makes it idea for microarry analysis. To clone the mechanosensory receptor specifically or highly expressed in the primary sensory neurons innervating pennis glan. There is a specific mechanosensory receptor in the primary sensory neurons innervating pennis glan. 1. Retrograde labeling the innervating nerve by DiI 2. Dissect DRGs, dissociate neurons and pick up the fluorescent cells. 3. Pool around 20 positive cells and 50 control cells (big diameter neuron and small to medium diameter neuron respectively), purify total RNA. 4. Two rounds of amplification 5. Affymetrix analysis Keywords: dorsal root ganglion, touch sensatiion

2008-04-19 | GSE11207 | GEO

Transcriptional Programming of Human Mechanosensory Neuron Subtypes from Pluripotent Stem Cells

Project description:In vitro generation of human peripheral sensory neurons may provide a framework for novel drug screening platforms and disease models of touch and pain. However, derivation of a functionally pure sensory neuron population remains a major unmet challenge. We discovered that, by expressing the transcription factors NGN2 and BRN3A, human pluripotent stem cells can be induced to differentiate into a surprisingly homogenous culture of cold- and mechano-sensing neurons. Although such a neuronal subtype has not been reported in mice, we found molecular evidence of its existence in adult human sensory ganglia. Combining NGN2 and BRN3A programming with neural crest patterning, we produced two additional populations of sensory neurons, including a more specialized mechanosensory neuron subtype. Finally, we applied this system to model a rare inherited sensory disorder, characterized by profound impairment of touch sensation and proprioception, caused by inactivating mutations in PIEZO2. Together these findings establish an approach to specify distinct sensory neuron subtypes in vitro, underscoring the utility of stem cell technology to capture human-specific features of physiology and disease.

2020-01-21 | GSE139273 | GEO

Transcriptional Programming of Human Mechanosensory Neuron Subtypes from Pluripotent Stem Cells

2019-12-31 | GSE139409 | GEO