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Transcriptome analysis Arabidopsis Col-0 plants grown in presence of SynComCol-0, SynCommfec and under axenic condition in Gnotopot growth system

ABSTRACT: We performed RNA-Seq based gene expression analysis of Arabidopsis Col-0 plants grown in presence of SynComCol-0 (eubiotic bacterial community), SynCommfec (dysbiotic bacterial community) and Axenic conditions in GnotoPot plant gnotobiotic growth system. SynCom preparation was done by mixing equal ratio of the each strain measured based on optical density of (OD600) in 10 mM MgCl2 and adjusting to the final combined OD600 of 0.04. Plants were grow in GnotoPots as described in (Chen et al, Nature 2020). We identified genes differentially enriched in response to presence of eubiotic and dysbiotic bacterial communities. Our results suggested that in presence of dysbiotic community there is over abundance of gene expression for immunity/defense-related genes in SynCommfec compared SynComCol-0 colonized plants.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE218962 | GEO | 2023/07/19

REPOSITORIES: GEO

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