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Transcriptome analysis Arabidopsis Col-0 plants grown in gnotobiotic growth system

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE218963 | GEO | 2023/07/19

REPOSITORIES: GEO

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Transcriptome analysis Arabidopsis Col-0 plants grown under axenic and holoxenic conditions in FlowPot gnotobiotic growth system

Project description:We performed RNA-Seq based gene expression analysis of Arabidopsis Col-0 plants grown under axenic and holoxenic conditions in FlowPot system. Holoxenic plants were grown in the presence of soil slurries containing microbial communities derived from natural soils and under axenic condition in presence of heat-killed soil slurries for three weeks. We identified genes differentially enriched in response to presence of microbial communities. Our results suggested that in presence of microbiota there is a differential expression of immunity/defense-related genes in holoxenic compared to axenic plants.

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Transcriptome analysis Arabidopsis Col-0 plants grown in gnotobiotic growth system

Project description:Transcriptome analysis Arabidopsis Col-0 plants grown in gnotobiotic growth system

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Transcriptome analysis Arabidopsis Col-0 plants grown in presence of SynComCol-0, SynCommfec and under axenic condition in Gnotopot growth system

Project description:We performed RNA-Seq based gene expression analysis of Arabidopsis Col-0 plants grown in presence of SynComCol-0 (eubiotic bacterial community), SynCommfec (dysbiotic bacterial community) and Axenic conditions in GnotoPot plant gnotobiotic growth system. SynCom preparation was done by mixing equal ratio of the each strain measured based on optical density of (OD600) in 10 mM MgCl2 and adjusting to the final combined OD600 of 0.04. Plants were grow in GnotoPots as described in (Chen et al, Nature 2020). We identified genes differentially enriched in response to presence of eubiotic and dysbiotic bacterial communities. Our results suggested that in presence of dysbiotic community there is over abundance of gene expression for immunity/defense-related genes in SynCommfec compared SynComCol-0 colonized plants.

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Transcriptome analysis Arabidopsis Col-0 plants grown under axenic and holoxenic conditions in FlowPot gnotobiotic growth system

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Transcriptome analysis Arabidopsis Col-0 plants grown in presence of SynComCol-0, SynCommfec and under axenic condition in Gnotopot growth system

Project description:Transcriptome analysis Arabidopsis Col-0 plants grown in presence of SynComCol-0, SynCommfec and under axenic condition in Gnotopot growth system

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