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MicroRNA expression in fibroblastic foci within idiopathic pulmonary fibrosis lungs reveals novel disease relevant pathways.

ABSTRACT: MicroRNAs (miRs) are a class of non-coding RNAs of ~22nt long that play an important role in regulating gene expression at a post-transcriptional level. Aberrant levels of miRs have been associated with profibrotic processes in idiopathic pulmonary fibrosis (IPF). However, the majority of these studies used whole IPF tissue or in vitro monocultures in which fibrosis has been artificially induced. In this study, we used laser microdissection to collect fibroblastic foci (FF), the key pathological lesion in IPF, then isolate miRs and compare their expression levels to those found in whole IPF lung tissue and/or in vitro cultured fibroblast from IPF or normal lungs. Methods – LCM was utilised on formalin embedded IPF tissue to isolate multiple FF and isolate miRs. Libraries were generated and sequenced; data were bioinformatically analysed and Ingenuity Pathway Analysis (IPA) used for integration. Results - Our analysis revealed a total of 49 miRs differentially expressed in FF, of which only 7 were also differentially expressed in in vitro cultured fibroblast. This approach uncovered novel miRs that could be related to IPF. Conclusion - miR expression analyses in LCM isolated FF were able to identify up to 40 miRs not previously described in IPF. Differential expression of these miRs could potentially contribute to IPF development and progression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE220107 | GEO | 2023/01/09

REPOSITORIES: GEO

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