Effect of depletion of RBM4 on gene expression of esophageal squamous cell carcinoma cell KYSE150
Ontology highlight
ABSTRACT: To explore the role of RBM4 in esophageal squamous cell carcinoma cell,we performed high-throughput mRNA sequencing (mRNA-seq) in KYSE150 with stable knockdown of RBM4 by shRNA . We then performed gene expression profiling analysis using data obtained from RNA-seq of KYSE150 cells,three replicates were sequenced.
Project description:In order to profile the transcription events that were regulated by RBM4, mRNA-seq was performed on KYSE150_shRBM4 and KYSE150_ctl which based on KYSE150 cells
Project description:We obtained transcriptome profiling (RNA-seq) of human esophageal squamous cell carcinoma cell line KYSE150 stabley transfected clones with pIRES2-EGFP vector or human NCCRP1-expression vector by using next generation sequencing.
Project description:We have mapped binding sites for the histone demethylase, JMJD2C/KDM4C/GASC1, and the effect of JMJD2C depletion on H3K9me3 and H3K36me3 distributions in KYSE150 cells. The human esophageal carcinoma cell line, KYSE150, contains an amplification of the JMJD2C locus. ChIP-seq was performed using chromatin from control or JMJD2C-depleted KYSE150 cells and antibodies recognizing JMJD2C, H3K4me3, H3K9me3 or H3K36me3.
Project description:RNA-seq was performed on A549 and H1299 cells that stably knocking down RBM4 or control, in order to profile the gene expression change that were regulated by RBM4
Project description:We have identified a new novel spliced variant of Lysyl Oxidase-like 2 (LOXL2), termed LOXL2 delta72, in human oesophageal carcinoma cells (ESCC). To explore the biological function of this variant, the cDNA microarrays was perform to assess the genes expression induced by its over-expression in ESCC KYSE150. We used microarrays to detail the global programmed of genes expression when LOXL2 delta72 over-expressed in KYSE150 cells compared with wild-type LOXL2.
Project description:In order to profile the transcription events that were regulated by RBM4, mRNA-seq was performed on A549_shRBM4 and A549_ctl which based on A549 cells , H1299_shRBM4 and H1299_ctl which based on H1299 cells
Project description:We have mapped binding sites for the histone demethylase, JMJD2C/KDM4C/GASC1, and the effect of JMJD2C depletion on H3K9me3 and H3K36me3 distributions in KYSE150 cells. The human esophageal carcinoma cell line, KYSE150, contains an amplification of the JMJD2C locus.