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Transcriptomic impact of microRNAs-449 or microRNAs-34 overexpression in proliferating human airway epithelial cells

ABSTRACT: We used microarrays to detail the global programme of gene expression that occurs in response to miR-449 or miR-34 overexpression in proliferating HAECs.

ORGANISM(S): Homo sapiens

PROVIDER: GSE22143 | GEO | 2011/06/07

SECONDARY ACCESSION(S): PRJNA129195

REPOSITORIES: GEO

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Transcriptomic impact of microRNAs-449 or microRNAs-34 overexpression in proliferating human airway epithelial cells

Project description:We used microarrays to detail the global programme of gene expression that occurs in response to miR-449 or miR-34 overexpression in proliferating HAECs. Each donors were transfected with pre-miR-Negative control, pre-miR-449a and pre-miR-449b. The donors 3 and 4 were morever transfected with pre-miRs-34 (34a, 34b-5p, 34c-5p).

2011-06-06 | E-GEOD-22143 | biostudies-arrayexpress

Gene expression changes in the respiratory epithelium of miR-34/449 knockout mice

Project description:MicroRNA ablation prevents multiciliogenesis via cell cycle deregulation MicroRNAs (miRNAs) have been implicated in various biological processes but have been most frequently described to inhibit proliferation and tumorigenesis. Here we describe an essential function of the miR-34/449 family during differentiation of multiciliated cells that is mediated by their well-described suppression of cell cycle progression. Constitutive deletion of all six members of this miRNA family triggers a derepression of multiple cell cycle-promoting proteins, thereby preventing epithelial cells from exiting the cell cycle and to maintain in a quiescent state. As a result, formation of motile multicilia is strongly inhibited in several tissues including the respiratory epithelium and the fallopian tube. Consequently, mice lacking miR-34/449 genes display infertility as well as severe chronic airway disease leading to postnatal death. These results show how miRNA-mediated repression of the cell cycle is required to allow multiciliogenesis to proceed during epithelial differentiation.

2017-09-12 | GSE92296 | GEO

microRNAs-449 control vertebrate multi-ciliogenesis by repressing Notch signalling

Project description:This SuperSeries is composed of the following subset Series: GSE22141: MicroRNA signature during the time course of regeneration of the human airway mucociliary epithelium GSE22142: Transcriptome analysis during the time course of regeneration of the human airway mucociliary epithelium GSE22143: Transcriptomic impact of microRNAs-449 or microRNAs-34 overexpression in proliferating human airway epithelial cells GSE22144: miRNAs high throughput sequencing profiling of regenerating human airway epithelial cells GSE22145: miRNAs high throughput sequencing profiling of basals cells and columnar cells GSE22146: microRNAs signatures of Xenopus laevis embryo epidermis at stage 11 (non ciliated) and 26 (ciliated) using high throughput sequencing Refer to individual Series

2011-06-06 | E-GEOD-22147 | biostudies-arrayexpress

Characterizing isomer variants in the microRNA-34/449 family

Project description:Characterizing isomer variants in the microRNA-34/449 family

| PRJNA309854 | ENA

Transcription profiling by array of mouse pachytene cells from mir-34bc+/-;449+/- and mir-34bc-/-;449-/- male animals.

Project description:Analysis of gene expression (mRNA profiles) from mouse pachytene cells. Mir-34bc+/-;449+/- samples are compared against mir-34bc-/-;449-/- samples .

2014-08-17 | E-MTAB-2668 | biostudies-arrayexpress

Transient transfection of miR-449 family in the hepatocellular carcinoma cell line HLE

2015-11-06 | GSE74710 | GEO

Analysis of Human Airway epithelial cells transcriptome after transfection of small RNAs from miR-34/449 family

Project description:Since the seed sequence is shifted between canonical and isomer variant of hsa-miR-34b-5p and hsa-miR-449c-5p, we examine whether the transfection of the different forms of these miRNAs results in distinct targetomes

2017-06-12 | GSE77251 | GEO

miR-34 regulates JARID1A expresion (NB1691)

Project description:p53 inactivation occurs only rarely in neuroblastoma, although miR-34, a transcriptional target of p53, is often deleted in neuroblastoma, suggesting another way in which p53 signaling might be impaired. In this study we show that miR-34 directly targets and downregulates the Polycomb Repressive Complex 2 (PRC2) and its associated histone demethylase, JARID1A, in a p53-dependent manner,

2014-01-01 | GSE49854 | GEO

MicroRNA mir-34 provides robustness to environmental stress response in C. elegans

Project description:Diverse stresses and aging result in changes in expression levels of microRNAs, suggesting a role for these posttrancriptional regulators of gene expression in stress modulation and longevity. Earlier studies demonstrated a central role for the miR-34 family in promoting cell cycle arrest and cell death following stress in human cells. However, the biological significance of this response was unclear, because wildtype and miR-34 family knockouts tested under a variety of stress conditions showed no difference in cell survival in vitro or in vivo. Here, we addressed this question in C. elegans by analyzing the effect of different stress conditions on phenotype, transcriptome and mir-34 expression in animals deficient in or overexpressing mir-34. We showed that mir-34 upregulation is necessary for developmental arrest, correct morphogenesis, and adaptation to a lower metabolic state to protect animals against stress-related damages. Either deletion or overexpression of mir-34 lead to impaired stress response, which can largely be explained by perturbations in a DAF-16/FOXO target genes. We demonstrate that mir-34 expression is regulated by the insulin signaling pathway via a negative feedback loop between miR-34 and DAF-16/FOXO. We propose that mir-34 provides robustness to stress response programs by controlling noise in the DAF-16/FOXO-regulated gene network.

2016-12-02 | GSE76413 | GEO

Transient transfection of miR-449 family in the hepatocellular carcinoma cell line HLE

Project description:In this study, we comparatively analyzed the members of the miR-449 family (miR-449a, miR-449b, and miR-449c) with regards to their target genes and functional effects in hepatocellular carcinoma (HCC). Microarray analysis after transient transfection of miR-449a, miR-449b, and/or miR-449c in the HCC cell line HLE identified putative target genes of miR-449a, miR-449b, and miR-449c. For transient overexpression of miRNAs, the HCC cell line HLE was transfected with 50 nM Allstars Negative Control or miScript miRNA mimics. MiRNA mimics for miR-449a, miR-449b, and miR-449c were either single transfected or cotransfected in equimolar amounts (16.7 nM each). Global mRNA expression profiling was performed utilizing pooled RNA of three biological replicates per microarray and two microarrays per condition.

2015-11-06 | E-GEOD-74710 | biostudies-arrayexpress

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